BMRB Entry 21011

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21011

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Title:
LPS bound structure of Cysteine deleted TachyplesinI
Deposition date:
2011-06-10
Original release date:
2018-12-19
Authors:
Bhattacharjya, Surajit; Saravanan, Rathi
Citation:

Citation: Saravanan, Rathi; Mohanram, Harini; Joshii, Mangeshi; Domadiai, Prerna N.; Torresi, Jaume; Ruedl, Christianei; Bhattacharjya, Surajit. "Structure, activity and interactions of the cysteine deleted analog of tachyplesin-1 with lipopolysaccharide micelle: Mechanistic insights into outer-membrane permeabilization and endotoxin neutralization."  Biochim. Biophys. Acta 1818, 1613-1624 (2012).
PubMed: 22464970

Assembly members:

Assembly members:
CDT, polymer, 13 residues, 1862.254 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CDT: KWFRVYRGIYRRR

Data sets:
Data typeCount
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CDT1

Entities:

Entity 1, CDT 13 residues - 1862.254 Da.

1   LYSTRPPHEARGVALTYRARGGLYILETYR
2   ARGARGARG

Samples:

sample_1: CDT 500 uM; LPS 10 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker AMX 600 MHz