BMRB Entry 2071

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR2071

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Title:
Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
Deposition date:
1995-07-31
Original release date:
1995-07-31
Authors:
Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan
Citation:

Citation: Meiering, Elizabeth; Bycroft, Mark; Fersht, Alan. "Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR"  Biochemistry 30, 11348-11356 (1991).

Assembly members:

Assembly members:
barnase, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1390   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus amyloliquefaciens

Experimental source:

Experimental source:   Production method: not available

Entity Sequences (FASTA):

Entity Sequences (FASTA):
barnase: XXXXXXFXXVAXXLXXXXXX XXNXXXKXXXQXXXXXXXXG XXXXXXXXKXIGGDXFXNXX XXXXXXXXRTWREADINXXX GXXNSDRXLXXSXXXIXKTT XHYQTFXXK

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1barnase1

Entities:

Entity 1, barnase 109 residues - Formula weight is not available

1   XXXXXXPHEXXVAL
2   ALAXXLEUXXXXXX
3   XXASNXXXLYSXXX
4   GLNXXXXXXXXGLY
5   XXXXXXXXLYSX
6   ILEGLYGLYASPXPHEXASNXX
7   XXXXXXXXARGTHR
8   TRPARGGLUALAASPILEASNXXX
9   GLYXXASNSERASPARGXLEUX
10   XSERXXXILEXLYSTHRTHR
11   XHISTYRGLNTHRPHEXXLYS

Samples:

sample_one:

sample_condition_set_one: pH: 4.5 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz