BMRB Entry 20118

Name: A 15-residue peptide corresponding to the C-terminal domain of the Gq protein alpha subunit (Gaq-Ct peptide) was synthesized and characterized using NMR spectroscopic studies.
Published: 2011-02-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20118

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

A 15-residue peptide corresponding to the C-terminal domain of the Gq protein alpha subunit (Gaq-Ct peptide) was synthesized and characterized using NMR spectroscopic studies.

Deposition date:

2010-01-15

Original release date:

2011-02-14

Authors:

Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He

Citation:

Citation: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He. "Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity" Biochemistry 49, 6365-6374 (2010).
PubMed: 20590159

Assembly members:

Assembly members:
C-terminal_domain_of_the_Gq_protein_alpha_subunit, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: .

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal_domain_of_the_Gq_protein_alpha_subunit: KDTILQLNLKEYNLV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
conformer_family_coord_set
- ensemble_coordinates

Data type	Count
1H chemical shifts	88
conformer coordinate sets	1

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal domain of the Gq protein alpha subunit	1

Entities:

Entity 1, C-terminal domain of the Gq protein alpha subunit 15 residues - Formula weight is not available

1

LYS

ASP

THR

ILE

LEU

GLN

LEU

ASN

LEU

LYS

2

GLU

TYR

ASN

LEU

VAL

Samples:

sample_1: C-terminal domain of the Gq protein alpha subunit 1.1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

FELIX v2000, Accelrys Software Inc. - chemical shift assignment, chemical shift calculation, data analysis, peak picking, processing, refinement, structure solution

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	20109 20112
EMBL	CAG08422
GB	ACF47673 ACF47675 EGW10015 EGW13773 EHB02791
REF	XP_004048208 XP_004059801 XP_005431300 XP_005642455 XP_008508489