BMRB Entry 20092
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20092
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Citation: Harrisona, Rosemary; Shepherda, Nicholas; Hoanga, Huy; Ruiz-Gomeza, Gloria; Hilla, Timothy; Drivera, Russell; Desaib, Vishal; Youngb, Paul; Abbenantea, Giovanni; Fairliea, David. "Downsizing human, bacterial, and viral proteins to short water-stable alpha helices that maintain biological potency" Proc. Natl. Acad. Sci. U. S. A. 107, 11686-11691 (2010).
PubMed: 20543141
Assembly members:
csp, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Streptococcus pneumoniae CSP-1 Taxonomy ID: 1313 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus pneumoniae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
csp: EXRLKKFFDKFILDRKKX
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 89 |
| coupling constants | 14 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | csp | 1 |
Entities:
Entity 1, csp 18 residues - Formula weight is not available
Resiude X represents Nle (Norleucine)
| 1 | GLU | NLE | ARG | LEU | LYS | LYS | PHE | PHE | ASP | LYS | ||||
| 2 | PHE | ILE | LEU | ASP | ARG | LYS | LYS | NH2 |
Samples:
sample_1: csp2 – 5 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 4.1; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz