BMRB Entry 20086
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20086
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Citation: Harrison, Rosemary; Ruiz-Gomez, Gloria; Hill, Timothy; Chow, Shiao; Shepherd, Nicholas; Lohman, Rink-Jan; Abbenante, Giovanni; Hoang, Huy; Fairlie, David. "Novel Helix-Constrained Nociceptin Derivatives Are Potent Agonists and Antagonists of ERK Phosphorylation and Thermal Analgesia in Mice." J. Med. Chem. 53, 8400-8408 (2010).
PubMed: 21067234
Assembly members:
Nociceptin_Antagonist, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Nociceptin_Antagonist: FGGFTGKRKSDRKKANQD
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nociceptin Antagonist | 1 |
Entities:
Entity 1, Nociceptin Antagonist 18 residues - Formula weight is not available
| 1 | PHE | GLY | GLY | PHE | THR | GLY | LYS | ARG | LYS | SER | ||||
| 2 | ASP | ARG | LYS | LYS | ALA | ASN | GLN | ASP |
Samples:
sample_1: Nociceptin Antagonist 3 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.5; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz