BMRB Entry 20080

Name: Structure and interactions in membranes of human and bovine Catestatin by solution and solid-state NMR spectroscopy
Published: 2010-03-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20080

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure and interactions in membranes of human and bovine Catestatin by solution and solid-state NMR spectroscopy

Deposition date:

2009-04-09

Original release date:

2010-03-01

Authors:

Sugewara, Masae; Resende, Jarbas; Moraes, Cleria; Chich, Jean-Francois; Marquette, Arnaud; Metz-Boutigue, Marie-Helene; Bechinger, Burkhard

Citation:

Citation: Sugawara, Masae; Resende, Jarbas; Moraes, Cleria Mendonca; Marquette, Arnaud; Chich, Jean-Francois; Metz-Boutigue, Marie-Helene; Bechinger, Burkhard. "Membrane structure and interactions of human catestatin by multidimensional solution and solid-state NMR spectroscopy." FASEB J. 24, 1737-1746 (2010).
PubMed: 20103720

Assembly members:

Assembly members:
hCGA(G364S), polymer, 21 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hCGA(G364S): SSMKLSFRARAYSFRGPGPQ L

Data sets:

assigned_chemical_shifts
- G346S-cs.txt
conformer_family_coord_set
- ensemble_coordinates

Data type	Count
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hCGA(G364S)	1

Entities:

Entity 1, hCGA(G364S) 21 residues - Formula weight is not available

1

SER

MET

LYS

LEU

SER

PHE

ARG

ALA

ARG

2

ALA

TYR

SER

PHE

ARG

GLY

PRO

GLY

PRO

GLN

3

LEU

Samples:

sample_1: hCGA(G364S) 2.0 ± 0.1 mM; DPC-d38, [U-2H], 400 ± 1.0 mM; D2O, [U-2H], 95%; H2O 5.0%; PB 10 mM

sample_conditions_1: pH: 5.5; temperature: 298.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

Bruker Avance 500 MHz