BMRB Entry 20075
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20075
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Citation: Zhu, Shunyi; Gao, Bin; Aumelas, Andre; del Carmen Rodriguez, Maria; Lanz-Mendoza, Humberto; Peigneur, Steve; Diego-Garcia, Elia; Martin-Eauclaire, Marie-France; Tytgat, Jan; Possani, Lourival. "MeuTXKbeta1, a scorpion venom-derived two-domain potassium channel toxin-like peptide with cytolytic activity." Biochim. Biophys. Acta 1804, 872-883 (2010).
PubMed: 20045493
Assembly members:
MeuTXKbeta1, polymer, 21 residues, Formula weight is not available
Natural source: Common Name: lesser Asian scorpion Taxonomy ID: 34648 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesobuthus eupeus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MeuTXKbeta1: GFREKHFQRFVKYAVPESTL
R
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 161 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MeuTXKbeta1 | 1 |
Entities:
Entity 1, MeuTXKbeta1 21 residues - Formula weight is not available
| 1 | GLY | PHE | ARG | GLU | LYS | HIS | PHE | GLN | ARG | PHE | ||||
| 2 | VAL | LYS | TYR | ALA | VAL | PRO | GLU | SER | THR | LEU | ||||
| 3 | ARG |
Samples:
sample_1: MeuTXKbeta11.5 – 2.0 mM
sample_conditions_1: pH: 4.5; temperature: 286 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection, processing
DYANA, Guntert, Braun and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz