BMRB Entry 20051

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20051

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Biological and structural study of Obestatin and its analogues Structural importance of Obestatin and its biology

Deposition date:

2008-10-21

Original release date:

2011-06-02

Authors:

Subasinghage, Anusha; Hewage, Chandralal; Flatt, Peter; Green, Brian

Citation:

Citation: Subasinghage, Anusha; Green, Brian; Flatt, Peter; Irwin, Nigel; Hewage, Chandralal. "Metabolic and structural properties of human obestatin {1-23} and two fragment peptides." Peptides 31, 1697-1705 (2010).
PubMed: 20553778

Assembly members:

Assembly members:
Human_Obestatin, polymer, 23 residues, 2547.9 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human_Obestatin: FNAPFDVGIKLSGVQYQQHS QAL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	137

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human Obestatin	1

Entities:

Entity 1, Human Obestatin 23 residues - 2547.9 Da.

1

PHE

ASN

ALA

PRO

PHE

ASP

VAL

GLY

ILE

LYS

2

LEU

SER

GLY

VAL

GLN

TYR

GLN

HIS

SER

3

GLN

ALA

LEU

Samples:

sample_1: Human Obestatin 1.5 mM

sample_conditions_1: pH: 3.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - processing

SPARKY v3.110, Goddard - peak picking

SYBYL v6.8.1, Tripos - refinement

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

Bruker DRX 500 MHz

DBJ	BAA89371 BAB19045 BAG16755 BAG16756 BAG16757
EMBL	CAB65733
GB	AAG10300 AAH25791 AAQ74381 AAQ74837 AAQ89412
REF	NP_001028075 NP_001128413 NP_001128416 NP_001128417 NP_001128418
SP	A0MLS4 Q9UBU3
AlphaFold	A0MLS4 Q9UBU3