BMRB Entry 20050
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20050
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Citation: Fletcher, Jordan; Morton, Craig; Zwar, Richard; Murray, Simon; O'Leary, Paul; Hughes, Richard. "Design of a conformationally defined and proteolytically stable circular mimetic of brain-derived neurotrophic factor" J. Biol. Chem. 283, 33375-33383 (2008).
PubMed: 18809686
Assembly members:
D-PAKKR, polymer, 5 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
D-PAKKR: XAKKR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 41 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | D-PAKKR monomer | 1 |
Entities:
Entity 1, D-PAKKR monomer 5 residues - Formula weight is not available
Residue 1 is a D-proline. The peptide is cyclised so that the C-terminus (Arg) links back to the N-terminus (D-Pro).
| 1 | DPR | ALA | LYS | LYS | ARG |
Samples:
sample_1: D-PAKKR 2-5 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.2; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
DYANA, Guntert, Braun and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 400 MHz