BMRB Entry 20047
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20047
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Citation: Melo, Manuel; Sousa, Francisco; Carneiro, Fabiana; Castanho, Miguel; Valente, Ana Paula; Almeida, Fabio; da Poian, Andrea; Mohana-Borges, Ronaldo. "Interaction of the dengue virus fusion peptide with membranes assessed by NMR: The essential role of the envelope protein TRP101 for membrane fusion." J. Mol. Biol. 392, 736-746 (2009).
PubMed: 19619560
Assembly members:
fusionpeptide, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: Dengue virus Taxonomy ID: 12637 Superkingdom: Viruses Kingdom: not available Genus/species: Flavivirus Dengue virus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
fusionpeptide: DRGWGNGCGLFGKGG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | fusiopeptide | 1 |
Entities:
Entity 1, fusiopeptide 15 residues - Formula weight is not available
This is the fusion peptide of dengue glycoprotein E
| 1 | ASP | ARG | GLY | TRP | GLY | ASN | GLY | CYS | GLY | LEU | ||||
| 2 | PHE | GLY | LYS | GLY | GLY |
Samples:
DPC_micelles: fusionpeptide 2 mM; DPC, [U-99% 2H], 100 mM; DTT, [U-99% 2H], 10 mM; sodium phosphate 100 mM; sodium azide 0.01%; D2O, [U-99% 2H], 10%
sample_conditions: pH: 5.5; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | DPC_micelles | isotropic | sample_conditions |
| 2D 1H-1H NOESY | DPC_micelles | isotropic | sample_conditions |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, peak picking
CNSSOLVE v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
ProcheckNMR, Laskowski and MacArthur - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA00176 BAA00254 BAA00255 BAA00393 BAA00394 |
| EMBL | CAA27184 CAA27332 CAA33284 CAA33474 CAA33475 |
| GB | AAA17498 AAA17499 AAA17500 AAA17501 AAA17502 |
| PIR | C32401 GNWVDF GNWVS5 |
| PRF | 1303269A 1412347A 1611496A 1613132A 1613132B |
| REF | NP_041724 NP_041726 NP_051124 NP_056776 NP_059433 |
| SP | P03314 P05769 P06935 P07564 P09732 |
| AlphaFold | P03314 P05769 P06935 P07564 P09732 |