BMRB Entry 20026
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20026
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tan, Y.; Jiang, L.; Wang, M.L.; Yin, F.F.; Deng, F.; Liu, M.L.; Hu, Z.H.; Wang, H.L.. "Mutagenesis and nuclear magnetic resonance analyses of the fusion peptide of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus F protein" J. Virol. 82, 8138-8148 (2008).
PubMed: 18524820
Assembly members:
F protein, polymer, 20 residues, 2144.487 Da.
Natural source: Common Name: cotton bollworm Taxonomy ID: 29058 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Helicoverpa armigera
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
F protein: NIGLNFVGSVDKFLFGVMDA
- assigned_chemical_shifts
- conformer_family_coord_set
- representative_conformer
| Data type | Count |
| 1H chemical shifts | 137 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | F protein | 1 |
Entities:
Entity 1, F protein 20 residues - 2144.487 Da.
| 1 | ASN | ILE | GLY | LEU | ASN | PHE | VAL | GLY | SER | VAL | |
| 2 | ASP | LYS | PHE | LEU | PHE | GLY | VAL | MET | ASP | ALA |
Samples:
sample_1: entity 2 mM; sodium phosphate 20 mM; SDS, [U-100% 2H], 200 mM
sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz