BMRB Entry 20023

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20023

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Title:
Structure, dynamics and selectivity of the sodium channel blocker mu-conotoxin SIIIA
Deposition date:
2008-05-28
Original release date:
2009-03-02
Authors:
Yao, Shenggen; Zhang, Minmin; Yoshikami, Doju; Azam, Layla; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond
Citation:

Citation: Yao, Shenggen; Zhang, Min-Min; Yoshikami, Doju; Azam, Layla; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond. "Structure, dynamics and selectivity of the sodium channel blocker mu-conotoxin SIIIA"  Biochemistry 47, 10940-10949 (2008).
PubMed: 18798648

Assembly members:

Assembly members:
SIIIA, polymer, 21 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: striated cone   Taxonomy ID: 6493   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus striatus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SIIIA: XNCCNGGCSSKWCRDHARCC X

Data sets:
Data typeCount
1H chemical shifts111
13C chemical shifts52
15N chemical shifts26

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SIIIA1

Entities:

Entity 1, SIIIA 21 residues - Formula weight is not available

1   PCAASNCYSCYSASNGLYGLYCYSSERSER
2   LYSTRPCYSARGASPHISALAARGCYSCYS
3   NH2

Samples:

sample_1: SIIIA 0.9 ± 0.1 mM

sample_2: SIIIA 0.9 ± 0.1 mM

sample_conditions_1: temperature: 298 K; pH: 4.2

sample_conditions_2: temperature: 283 K; pH: 4.2

sample_conditions_3: temperature: 310 K; pH: 4.2

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
2D 1H-13C HMQC-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

XEASY v1.3, Bartels et al. - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 20025
GB AAO48588 AEX60326 AEX60330 AEX60428
SP Q86DU6
AlphaFold Q86DU6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks