BMRB Entry 20018
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20018
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Citation: Abbassi, Feten; Galanth, Cecile; Amiche, Mohamed; Saito, Kazuko; Piesse, Christophe; Zargarian, Loussine; Hani, Khaled; Nicolas, Pierre; Lequin, Olivier; Ladram, Ali. "Solution structure and model membrane interactions of temporins-SH,
antimicrobial peptides from amphibian skin. A NMR spectroscopy and differential
scanning calorimetry study" Biochemistry 47, 10513-10525 (2008).
PubMed: 18795798
Assembly members:
Temporin-SHc, polymer, 14 residues, 1466.719 Da.
Natural source: Common Name: Rana saharica Taxonomy ID: 70019 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana saharica
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Temporin-SHc: FLSHIAGFLSNLFX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 52 |
| 1H chemical shifts | 100 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Temporin-SHc | 1 |
Entities:
Entity 1, Temporin-SHc 14 residues - 1466.719 Da.
| 1 | PHE | LEU | SER | HIS | ILE | ALA | GLY | PHE | LEU | SER | ||||
| 2 | ASN | LEU | PHE | NH2 |
Samples:
sample_1: entity 1.5 mM; SDS, [U-99% 2H], 80 mM; DSS 0.1 mM
sample_conditions_1: pH: 5; temperature: 309.5 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz