BMRB Entry 20017

Name: NMR structure of Temporin-SHb in micellar SDS
Published: 2009-01-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20017

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of Temporin-SHb in micellar SDS

Deposition date:

2008-04-09

Original release date:

2009-01-16

Authors:

Abbassi, Feten; Galanth, Cecile; Lequin, Olivier; Saito, Kazuko; Piesse, Christophe; Zargarian, Loussin; Hani, Khaled; Nicolas, Pierre; Amiche, Mohamed; Ladram, Ali

Citation:

Citation: Abbassi, Feten; Galanth, Cecile; Amiche, Mohamed; Saito, Kazuko; Piesse, Christophe; Zargarian, Loussine; Hani, Khaled; Nicolas, Pierre; Lequin, Olivier; Ladram, Ali. "Solution structure and model membrane interactions of temporins-SH, antimicrobial peptides from amphibian skin. A NMR spectroscopy and differential scanning calorimetry study" Biochemistry 47, 10513-10525 (2008).
PubMed: 18795798

Assembly members:

Assembly members:
Temporin-SHb, polymer, 14 residues, 1399.732 Da.

Natural source:

Natural source: Common Name: Rana saharica Taxonomy ID: 70019 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana saharica

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Temporin-SHb: FLPIVTNLLSGLLX

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	51
1H chemical shifts	110

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Temporin-SHb	1

Entities:

Entity 1, Temporin-SHb 14 residues - 1399.732 Da.

1

PHE

LEU

PRO

ILE

VAL

THR

ASN

LEU

SER

2

GLY

LEU

NH2

Samples:

sample_1: entity 1.5 mM; SDS, [U-99% 2H], 80 mM; DSS 0.1 mM

sample_conditions_1: pH: 5; temperature: 309.5 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz