BMRB Entry 20011
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20011
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Citation: Honda, Shinya; Akiba, Toshihiko; Kato, Yusuke; Sawada, Yoshito; Sekijima, Masakazu; Ishimura, Miyuki; Ooishi, Ayako; Watanabe, Hideki; Odahara, Takayuki; Harata, Kazuaki. "Crystal structure of a ten-amino acid protein" J. Am. Chem. Soc. 130, 15327-15331 (2008).
PubMed: 18950166
Assembly members:
CLN025, polymer, 10 residues, 1294.336 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
CLN025: YYDPETGTWY
- conformer_family_coord_set
| Data type | Count |
| conformer coordinate sets | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CLN025 | 1 |
Entities:
Entity 1, CLN025 10 residues - 1294.336 Da.
Mutant of Cignolin, CLN025
| 1 | TYR | TYR | ASP | PRO | GLU | THR | GLY | THR | TRP | TYR |
Samples:
sample_1: CLN025 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; DSS 0.01%; H2O 95%; D2O 5%
sample_conditions_1: pH: 5.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
ProcheckNMR, Laskowski and MacArthur - data analysis
VNMR, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz