BMRB Entry 20008
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20008
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Citation: Dutertre, Sebastien; Croker, Daniel; Daly, Norelle; Andersson, Asa; Muttenthaler, Markus; Lumsden, Natalie; Craik, David; Alewood, Paul; Guillon, Gilles; Lewis, Richard. "Conopressin-T from Conus tulipa reveals an antagonist switch in vasopressin-like peptides" J. Biol. Chem. 283, 7100-7108 (2008).
PubMed: 18174156
Assembly members:
L7P-Con-T, polymer, 9 residues, Formula weight is not available
Natural source: Common Name: tulip cone Taxonomy ID: 6495 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus tulipa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
L7P-Con-T: CYIQNCPRV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 44 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | L7P Con-T | 1 |
Entities:
Entity 1, L7P Con-T 9 residues - Formula weight is not available
| 1 | CYS | TYR | ILE | GLN | ASN | CYS | PRO | ARG | VAL |
Samples:
sample_1: L7P-Con-T 1 mM
sample_conditions_1: pH: 3; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY, Goddard - data analysis
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz