BMRB Entry 20005

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20005

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Title:
NMR Solution Structure of Leucine-Enkephlain in DMPC+CHAPS (1:4) bicelles in 90% H2O/10% D2O of pH 6.6 at 298K
Deposition date:
2007-12-27
Original release date:
2008-05-27
Authors:
Mukhopadhyay, Chaitali; Gayen, Anindita
Citation:

Citation: Gayen, Anindita; Mukhopadhyay, Chaitali. "Evidence for Effect of GM1 on Opioid Peptide Conformation: NMR Study on Leucine Enkephalin in Ganglioside-Containing Isotropic Phospholipid Bicelles"  Langmuir 24, 5422-5432 (2008).
PubMed: 18412380

Assembly members:

Assembly members:
Leu-Enkephalin, polymer, 5 residues, 555.62 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Leu-Enkephalin: YGGFL

Data sets:
Data typeCount
13C chemical shifts24
1H chemical shifts28
coupling constants2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Leu-enkephalin1

Entities:

Entity 1, Leu-enkephalin 5 residues - 555.62 Da.

TYROSINE 1 GLYCINE 2 GLYCINE 3 PHENYLALANINE 4 LEUCINE 5

1   TYRGLYGLYPHELEU

Samples:

sample: Leu-Enkephalin 4-5 mM; DMPC+CHAPS+GM1 4 %w/v

bicelles: pH: 6.6 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsampleisotropicbicelles
2D 1H-1H NOESYsampleisotropicbicelles
2D DQF-COSYsampleisotropicbicelles
2D 1H-13C HSQCsampleisotropicbicelles

Software:

xwinnmr v3.75, Bruker Biospin, Goddard, Schwieters, Kuszewski, Tjandra and Clore - collection, data analysis, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

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