BMRB Entry 20002

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20002

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Title:
Solution structure of RgIA
Deposition date:
2007-12-20
Original release date:
2008-06-30
Authors:
Daly, Norelle; Craik, David
Citation:

Citation: Clark, Richard; Daly, Norelle; Halai, Reena; Nevin, Simon; Adams, David; Craik, David. "The three-dimensional structure of the analgesic alpha-conotoxin, RgIA"  FEBS Lett. 582, 597-602 (2008).
PubMed: 18242183

Assembly members:

Assembly members:
RgIA, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Conus regius   Taxonomy ID: 101314   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus regius

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RgIA: GCCSDPRCRYRCR

Data sets:
Data typeCount
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RgIA1

Entities:

Entity 1, RgIA 13 residues - Formula weight is not available

1   GLYCYSCYSSERASPPROARGCYSARGTYR
2   ARGCYSARG

Samples:

sample_1: RgIA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.5; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - data analysis

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz