BMRB Entry 19968

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19968

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Title:
1H, 13C, and 15N resonance assignments for the tandem PHD finger motifs of human CHD4
Deposition date:
2014-05-12
Original release date:
2016-10-06
Authors:
Walport, Louise; Morra, Rosa; Mancini, Erika; Redfield, Christina
Citation:

Citation: Walport, Louise; Morra, Rosa; Mancini, Erika; Redfield, Christina. "(1)H, (13)C, and (15)N resonance assignments for the tandem PHD finger motifs of human CHD4"  Biomol. NMR Assign. 9, 239-242 (2015).
PubMed: 25326197

Assembly members:

Assembly members:
dPHD, polymer, 148 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTriEx2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dPHD: METDHQDYCEVCQQGGEIIL CDTCPRAYHMVCLDPDMEKA PEGKWSCPHCEKEGIQWEAK EDNSEGEEILEEVGGDPEEE DDHHMEFCRVCKDGGELLCC DTCPSSYHIHCLNPPLPEIP NGEWLCPRCTCPALKGKKLE HHHHHHHH

Data sets:
Data typeCount
13C chemical shifts474
15N chemical shifts128
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dPHD1
2Zinc12
3Zinc22
4Zinc32
5Zinc42

Entities:

Entity 1, dPHD 148 residues - Formula weight is not available

Residues 130-7 represent a non-native affinity tag

1   METGLUTHRASPHISGLNASPTYRCYSGLU
2   VALCYSGLNGLNGLYGLYGLUILEILELEU
3   CYSASPTHRCYSPROARGALATYRHISMET
4   VALCYSLEUASPPROASPMETGLULYSALA
5   PROGLUGLYLYSTRPSERCYSPROHISCYS
6   GLULYSGLUGLYILEGLNTRPGLUALALYS
7   GLUASPASNSERGLUGLYGLUGLUILELEU
8   GLUGLUVALGLYGLYASPPROGLUGLUGLU
9   ASPASPHISHISMETGLUPHECYSARGVAL
10   CYSLYSASPGLYGLYGLULEULEUCYSCYS
11   ASPTHRCYSPROSERSERTYRHISILEHIS
12   CYSLEUASNPROPROLEUPROGLUILEPRO
13   ASNGLYGLUTRPLEUCYSPROARGCYSTHR
14   CYSPROALALEULYSGLYLYSLYSLEUGLU
15   HISHISHISHISHISHISHISHIS

Entity 2, Zinc1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: dPHD, [U-99% 15N], 1 mM; sodium chloride 200 mM; DTT 10 mM; TRIS 20 mM; D2O 5%; H2O 95%

sample_2: dPHD, [U-99% 13C; U-99% 15N], 1 mM; DTT 10 mM; sodium chloride 200 mM; TRIS 20 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 0.2 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D CBCANHsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D (H)C(CO)NHsample_2isotropicsample_conditions_1
3D NH(CA)COsample_2isotropicsample_conditions_1

Software:

NMRPipe vJune 2006 Sun Solaris, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis v2.1, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • home built OMEGA 750 MHz
  • home built OMEGA 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks