BMRB Entry 19952

Name: Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)
Published: 2014-05-20

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PDB ID: 2mou
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19952
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)

Deposition date:

2014-05-05

Original release date:

2014-05-20

Authors:

Letourneau, Danny; Bedard, Mikael; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre

Citation:

Citation: Letourneau, Danny; Bedard, Mikael; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre. "Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)" .

Assembly members:

Assembly members:
StAR_D6, polymer, 220 residues, 25037.572 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
StAR_D6: MDFKAIAQQTAQEVLGYNRD TSGWKVVKTSKKITVSSKAS RKFHGNLYRVEGIIPESPAK LSDFLYQTGDRITWDKSLQV YNMVHRIDSDTFICHTITQS FAVGSISPRDFIDLVYIKRY EGNMNIISSKSVDFPEYPPS SNYIRGYNHPCGFVCSPMEE NPAYSKLVMFVQTEMRGKLS PSIIEKTMPSNLVNFILNAK DGIKAHRTPSRRGFHHNSHS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
spectral_peak_list

Data type	Count
13C chemical shifts	878
15N chemical shifts	201
1H chemical shifts	1355

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	StAR D6	1

Entities:

Entity 1, StAR D6 220 residues - 25037.572 Da.

1	MET	ASP	PHE	LYS	ALA	ILE	ALA	GLN	GLN	THR
2	ALA	GLN	GLU	VAL	LEU	GLY	TYR	ASN	ARG	ASP
3	THR	SER	GLY	TRP	LYS	VAL	VAL	LYS	THR	SER
4	LYS	LYS	ILE	THR	VAL	SER	SER	LYS	ALA	SER
5	ARG	LYS	PHE	HIS	GLY	ASN	LEU	TYR	ARG	VAL
6	GLU	GLY	ILE	ILE	PRO	GLU	SER	PRO	ALA	LYS
7	LEU	SER	ASP	PHE	LEU	TYR	GLN	THR	GLY	ASP
8	ARG	ILE	THR	TRP	ASP	LYS	SER	LEU	GLN	VAL
9	TYR	ASN	MET	VAL	HIS	ARG	ILE	ASP	SER	ASP
10	THR	PHE	ILE	CYS	HIS	THR	ILE	THR	GLN	SER
11	PHE	ALA	VAL	GLY	SER	ILE	SER	PRO	ARG	ASP
12	PHE	ILE	ASP	LEU	VAL	TYR	ILE	LYS	ARG	TYR
13	GLU	GLY	ASN	MET	ASN	ILE	ILE	SER	SER	LYS
14	SER	VAL	ASP	PHE	PRO	GLU	TYR	PRO	PRO	SER
15	SER	ASN	TYR	ILE	ARG	GLY	TYR	ASN	HIS	PRO
16	CYS	GLY	PHE	VAL	CYS	SER	PRO	MET	GLU	GLU
17	ASN	PRO	ALA	TYR	SER	LYS	LEU	VAL	MET	PHE
18	VAL	GLN	THR	GLU	MET	ARG	GLY	LYS	LEU	SER
19	PRO	SER	ILE	ILE	GLU	LYS	THR	MET	PRO	SER
20	ASN	LEU	VAL	ASN	PHE	ILE	LEU	ASN	ALA	LYS
21	ASP	GLY	ILE	LYS	ALA	HIS	ARG	THR	PRO	SER
22	ARG	ARG	GLY	PHE	HIS	HIS	ASN	SER	HIS	SER

Samples:

STARD6: StAR_D6, [U-100% 13C; U-100% 15N], 1 mM

CondSet1: ionic strength: 0.100 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.150 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	STARD6	isotropic	CondSet1
3D C(CO)NH	STARD6	isotropic	CondSet1
3D HNCO	STARD6	isotropic	CondSet1
2D 1H-15N HSQC	STARD6	isotropic	CondSet1
3D HN(CO)CA	STARD6	isotropic	CondSet1
3D HNHA	STARD6	isotropic	CondSet1
3D H(CCO)NH	STARD6	isotropic	CondSet1
3D 1H-15N NOESY	STARD6	isotropic	CondSet1
3D HCCH-TOCSY	STARD6	isotropic	CondSet1
2D 1H-13C HSQC	STARD6	isotropic	CondSet1
3D 1H-13C NOESY aliphatic	STARD6	isotropic	CondSet1
2D 1H-13C HSQC aromatic	STARD6	isotropic	CondSet1
3D 1H-13C NOESY aromatic	STARD6	isotropic	CondSet1
2D 1H-13C HSQC aliphatic	STARD6	isotropic	CondSet1

Software:

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - Structure calculation, refinement

CcpNmr_Analysis v2.2.2, CCPN - Assignment

DANGLE v1.1, CCPN - Dihedral Restraints from Chemical Shifts

NMR_Pipe v2.0, NMR Pipe - file conversion

ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - Structure calculation, refinement

NMR spectrometers:

Varian UnityInova 600 MHz

UniProt	P59095
BMRB	18777
PDB	2MOU
DBJ	BAI46405
GB	AAI40309 AAI46469 AAL89655 EAW63002 EAW63004
REF	NP_631910 XP_011524122 XP_011524123 XP_014197853 XP_523930
SP	P59095
AlphaFold	P59095 P59095

1	MET	ASP	PHE	LYS	ALA	ILE	ALA	GLN	GLN	THR
2	ALA	GLN	GLU	VAL	LEU	GLY	TYR	ASN	ARG	ASP
3	THR	SER	GLY	TRP	LYS	VAL	VAL	LYS	THR	SER
4	LYS	LYS	ILE	THR	VAL	SER	SER	LYS	ALA	SER
5	ARG	LYS	PHE	HIS	GLY	ASN	LEU	TYR	ARG	VAL
6	GLU	GLY	ILE	ILE	PRO	GLU	SER	PRO	ALA	LYS
7	LEU	SER	ASP	PHE	LEU	TYR	GLN	THR	GLY	ASP
8	ARG	ILE	THR	TRP	ASP	LYS	SER	LEU	GLN	VAL
9	TYR	ASN	MET	VAL	HIS	ARG	ILE	ASP	SER	ASP
10	THR	PHE	ILE	CYS	HIS	THR	ILE	THR	GLN	SER
11	PHE	ALA	VAL	GLY	SER	ILE	SER	PRO	ARG	ASP
12	PHE	ILE	ASP	LEU	VAL	TYR	ILE	LYS	ARG	TYR
13	GLU	GLY	ASN	MET	ASN	ILE	ILE	SER	SER	LYS
14	SER	VAL	ASP	PHE	PRO	GLU	TYR	PRO	PRO	SER
15	SER	ASN	TYR	ILE	ARG	GLY	TYR	ASN	HIS	PRO
16	CYS	GLY	PHE	VAL	CYS	SER	PRO	MET	GLU	GLU
17	ASN	PRO	ALA	TYR	SER	LYS	LEU	VAL	MET	PHE
18	VAL	GLN	THR	GLU	MET	ARG	GLY	LYS	LEU	SER
19	PRO	SER	ILE	ILE	GLU	LYS	THR	MET	PRO	SER
20	ASN	LEU	VAL	ASN	PHE	ILE	LEU	ASN	ALA	LYS
21	ASP	GLY	ILE	LYS	ALA	HIS	ARG	THR	PRO	SER
22	ARG	ARG	GLY	PHE	HIS	HIS	ASN	SER	HIS	SER

1	MET	ASP	PHE	LYS	ALA	ILE	ALA	GLN	GLN	THR
2	ALA	GLN	GLU	VAL	LEU	GLY	TYR	ASN	ARG	ASP
3	THR	SER	GLY	TRP	LYS	VAL	VAL	LYS	THR	SER
4	LYS	LYS	ILE	THR	VAL	SER	SER	LYS	ALA	SER
5	ARG	LYS	PHE	HIS	GLY	ASN	LEU	TYR	ARG	VAL
6	GLU	GLY	ILE	ILE	PRO	GLU	SER	PRO	ALA	LYS
7	LEU	SER	ASP	PHE	LEU	TYR	GLN	THR	GLY	ASP
8	ARG	ILE	THR	TRP	ASP	LYS	SER	LEU	GLN	VAL
9	TYR	ASN	MET	VAL	HIS	ARG	ILE	ASP	SER	ASP
10	THR	PHE	ILE	CYS	HIS	THR	ILE	THR	GLN	SER
11	PHE	ALA	VAL	GLY	SER	ILE	SER	PRO	ARG	ASP
12	PHE	ILE	ASP	LEU	VAL	TYR	ILE	LYS	ARG	TYR
13	GLU	GLY	ASN	MET	ASN	ILE	ILE	SER	SER	LYS
14	SER	VAL	ASP	PHE	PRO	GLU	TYR	PRO	PRO	SER
15	SER	ASN	TYR	ILE	ARG	GLY	TYR	ASN	HIS	PRO
16	CYS	GLY	PHE	VAL	CYS	SER	PRO	MET	GLU	GLU
17	ASN	PRO	ALA	TYR	SER	LYS	LEU	VAL	MET	PHE
18	VAL	GLN	THR	GLU	MET	ARG	GLY	LYS	LEU	SER
19	PRO	SER	ILE	ILE	GLU	LYS	THR	MET	PRO	SER
20	ASN	LEU	VAL	ASN	PHE	ILE	LEU	ASN	ALA	LYS
21	ASP	GLY	ILE	LYS	ALA	HIS	ARG	THR	PRO	SER
22	ARG	ARG	GLY	PHE	HIS	HIS	ASN	SER	HIS	SER