BMRB Entry 19908

Title:
NMR resonance assignment of the archaeal ribosomal protein L7Ae bound to a 25 nt RNA   PubMed: 25030110
Deposition date:
2014-04-11
Original release date:
2019-07-12
Authors:
Moschen, Thomas; Wunderlich, Christoph; Kreutz, Christoph; Tollinger, Martin
Citation:

Citation: Moschen, Thomas; Wunderlich, Christoph; Kreutz, Christoph; Tollinger, Martin. "NMR resonance assignments of the archaeal ribosomal protein L7Ae in the apo form and bound to a 25 nt RNA"  Biomol. NMR Assign. 9, 177-180 (2015).

Assembly members:

Assembly members:
L7Ae_complex, polymer, 121 residues, 13123.3 Da.
25_nt_C/D-box_RNA_mimic, polymer, 25 residues, 8075.93 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts111
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L7Ae1
225nt C/D box mimic2

Entities:

Entity 1, L7Ae 121 residues - 13123.3 Da.

first 4 residues are left from cleavage with thrombin

1   ARGGLYSERHISMETALAVALTYRVALLYS
2   PHELYSVALPROGLUGLUILEGLNLYSGLU
3   LEULEUASPALAVALALALYSALAGLNLYS
4   ILELYSLYSGLYALAASNGLUVALTHRLYS
5   ALAVALGLUARGGLYILEALALYSLEUVAL
6   ILEILEALAGLUASPVALLYSPROGLUGLU
7   VALVALALAHISLEUPROTYRLEUCYSGLU
8   GLULYSGLYILEPROTYRALATYRVALALA
9   SERLYSGLNASPLEUGLYLYSALAALAGLY
10   LEUGLUVALALAALASERSERVALALAILE
11   ILEASNGLUGLYASPALAGLUGLULEULYS
12   VALLEUILEGLULYSVALASNVALLEULYS
13   GLN

Entity 2, 25nt C/D box mimic 25 residues - 8075.93 Da.

25 nucleotide C/D box mimic k-turn RNA

1   GCUCUGACCG
2   AAAGGCGUGA
3   UGAGC

Samples:

sample: L7Ae_complex, [U-100% 13C; U-100% 15N], 1 mM; 25 nt C/D-box RNA mimic 1 mM; D2O, [U-100% 2H], 10%; sodium cacodylate 10 mM; potassium chloride 50 mM; H2O 90%

sample_conditions: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsampleisotropicsample_conditions
2D 1H-13C HSQCsampleisotropicsample_conditions
3D HNCOsampleisotropicsample_conditions
3D HNCAsampleisotropicsample_conditions
3D HNCACBsampleisotropicsample_conditions
3D CBCA(CO)NHsampleisotropicsample_conditions
3D HN(CO)CAsampleisotropicsample_conditions
3D 15N-TOCSY-HSQCsampleisotropicsample_conditions

Software:

CCPN, CCPN - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Unity 500 MHz
  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts