BMRB Entry 19908

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19908

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Title:
NMR resonance assignment of the archaeal ribosomal protein L7Ae bound to a 25 nt RNA
Deposition date:
2014-04-11
Original release date:
2019-07-12
Authors:
Moschen, Thomas; Wunderlich, Christoph; Kreutz, Christoph; Tollinger, Martin
Citation:

Citation: Moschen, Thomas; Wunderlich, Christoph; Kreutz, Christoph; Tollinger, Martin. "NMR resonance assignments of the archaeal ribosomal protein L7Ae in the apo form and bound to a 25 nt RNA"  Biomol. NMR Assign. 9, 177-180 (2015).
PubMed: 25030110

Assembly members:

Assembly members:
L7Ae_complex, polymer, 121 residues, 13123.3 Da.
25_nt_C/D-box_RNA_mimic, polymer, 25 residues, 8075.93 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
L7Ae_complex: RGSHMAVYVKFKVPEEIQKE LLDAVAKAQKIKKGANEVTK AVERGIAKLVIIAEDVKPEE VVAHLPYLCEEKGIPYAYVA SKQDLGKAAGLEVAASSVAI INEGDAEELKVLIEKVNVLK Q
25_nt_C/D-box_RNA_mimic: GCUCUGACCGAAAGGCGUGA UGAGC

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts111
1H chemical shifts210

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1L7Ae1
225nt C/D box mimic2

Entities:

Entity 1, L7Ae 121 residues - 13123.3 Da.

first 4 residues are left from cleavage with thrombin

1   ARGGLYSERHISMETALAVALTYRVALLYS
2   PHELYSVALPROGLUGLUILEGLNLYSGLU
3   LEULEUASPALAVALALALYSALAGLNLYS
4   ILELYSLYSGLYALAASNGLUVALTHRLYS
5   ALAVALGLUARGGLYILEALALYSLEUVAL
6   ILEILEALAGLUASPVALLYSPROGLUGLU
7   VALVALALAHISLEUPROTYRLEUCYSGLU
8   GLULYSGLYILEPROTYRALATYRVALALA
9   SERLYSGLNASPLEUGLYLYSALAALAGLY
10   LEUGLUVALALAALASERSERVALALAILE
11   ILEASNGLUGLYASPALAGLUGLULEULYS
12   VALLEUILEGLULYSVALASNVALLEULYS
13   GLN

Entity 2, 25nt C/D box mimic 25 residues - 8075.93 Da.

25 nucleotide C/D box mimic k-turn RNA

1   GCUCUGACCG
2   AAAGGCGUGA
3   UGAGC

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