BMRB Entry 19902

Name: Solution NMR structure of the reovirus p15 fusion-associated small transmembrane (FAST) protein fusion-inducing lipid packing sensor (FLiPS) motif in dodecyl phosphocholine (DPC) micelles
Published: 2014-04-22

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PDB ID: 2mns
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19902
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the reovirus p15 fusion-associated small transmembrane (FAST) protein fusion-inducing lipid packing sensor (FLiPS) motif in dodecyl phosphocholine (DPC) micelles

Deposition date:

2014-04-09

Original release date:

2014-04-22

Authors:

Sarker, Muzaddid; Duncan, Roy; Read, Jolene; Rainey, Jan

Citation:

Citation: Read, Jolene; Clancy, Eileen; Sarker, Muzaddid; Langelaan, David; Rainey, Jan; Duncan, Roy. "Novel helix-loop-helix fusion-inducing lipid packing sensor (FLiPS) for cell-cell fusion" .

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, 1941.335 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XLGLLSYGAGVASLPLLNVI A

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	39
15N chemical shifts	20
1H chemical shifts	146

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 21 residues - 1941.335 Da.

1

ACE

LEU

GLY

LEU

SER

TYR

GLY

ALA

GLY

2

VAL

ALA

SER

LEU

PRO

LEU

ASN

VAL

ILE

3

ALA

Samples:

sample_1: entity_1 1 mM; DPC 150 mM; DSS 0.5 mM; sodium azide 0.2 mM; sodium acetate, [U-2H], 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR_NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - refinement

TOPSPIN v3.1, Bruker Biospin - processing

SPARKY v3.110, Goddard - chemical shift assignment

ProcheckNMR v3.5.4, Laskowski and MacArthur - validation

UCSF_CHIMERA v1.8, NIH - structure viewing

Molmol v2K.2, Koradi, Billeter and Wuthrich - ensemble viewing

NMR spectrometers:

Bruker Avance 700 MHz

PDB	2MNS
GB	AAL01373
REF	YP_004769555