BMRB Entry 19891
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19891
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Akimoto, Madoka; Zhang, Zaiyong; Boulton, Stephen; Selvaratnam, Rajeevan; VanSchouwen, Bryan; Gloyd, Melanie; Accili, Eric; Lange, Oliver; Melacini, Giuseppe. "A mechanism for the auto-inhibition of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel opening and its relief by cAMP" J. Biol. Chem. 289, 22205-22220 (2014).
PubMed: 24878962
Assembly members:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, polymer, 131 residues, 14985.341 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302NT-His
Entity Sequences (FASTA):
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4: NSEEIINFNCRKLVASMPLF
ANADPNFVTSMLTKLRFEVF
QPGDYIIREGTIGKKMYFIQ
HGVVSVLTKGNKETKLADGS
YFGEICLLTRGRRTASVRAD
TYCRLYSLSVDNFNEVLEEY
PMMRRAFETVA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 459 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 666 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 | 1 |
Entities:
Entity 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 131 residues - 14985.341 Da.
Residues 577N, 578S(First two residues) represent non-native amino acids from cleavage site.
| 1 | ASN | SER | GLU | GLU | ILE | ILE | ASN | PHE | ASN | CYS | ||||
| 2 | ARG | LYS | LEU | VAL | ALA | SER | MET | PRO | LEU | PHE | ||||
| 3 | ALA | ASN | ALA | ASP | PRO | ASN | PHE | VAL | THR | SER | ||||
| 4 | MET | LEU | THR | LYS | LEU | ARG | PHE | GLU | VAL | PHE | ||||
| 5 | GLN | PRO | GLY | ASP | TYR | ILE | ILE | ARG | GLU | GLY | ||||
| 6 | THR | ILE | GLY | LYS | LYS | MET | TYR | PHE | ILE | GLN | ||||
| 7 | HIS | GLY | VAL | VAL | SER | VAL | LEU | THR | LYS | GLY | ||||
| 8 | ASN | LYS | GLU | THR | LYS | LEU | ALA | ASP | GLY | SER | ||||
| 9 | TYR | PHE | GLY | GLU | ILE | CYS | LEU | LEU | THR | ARG | ||||
| 10 | GLY | ARG | ARG | THR | ALA | SER | VAL | ARG | ALA | ASP | ||||
| 11 | THR | TYR | CYS | ARG | LEU | TYR | SER | LEU | SER | VAL | ||||
| 12 | ASP | ASN | PHE | ASN | GLU | VAL | LEU | GLU | GLU | TYR | ||||
| 13 | PRO | MET | MET | ARG | ARG | ALA | PHE | GLU | THR | VAL | ||||
| 14 | ALA |
Samples:
sample_1: DTT 1 mM; MES 20 mM; potassium chloride 100 mM; EDTA 2 mM; EGTA 2 mM; sodium azide 0.02%; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, [U-13C; U-15N], 0.5 mM; D2O 95%; H2O 5%
sample_2: DTT 1 mM; MES 20 mM; potassium chloride 100 mM; EDTA 1 mM; sodium azide 0.02%; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, [U-15N], 0.2 mM; Pf1 phage 6 mg/mL; D2O 95%; H2O 5%
sample_3: DTT 1 mM; MES 20 mM; potassium chloride 100 mM; EDTA 1 mM; sodium azide 0.02%; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, [U-15N], 0.2 mM; D2O 95%; H2O 5%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | anisotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_3 | anisotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks