BMRB Entry 19887
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19887
MolProbity Validation Chart
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NMR-STAR v3 text file.
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RDF gzip file.
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Citation: Chirayil, Sara; Wu, Qiong; Amezcua, Carlos; Luebke, Kevin. "NMR Characterization of an Oligonucleotide Model of the MiR-21 Pre-Element" PLOS ONE 9, e108231-e108231 (2014).
PubMed: 25250627
Assembly members:
miR-21_pre-element, polymer, 29 residues, 111.103 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
miR-21_pre-element: GGGUUGACCGUUGAAUCUCA
CGGCAACCC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 100 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | miR-21 pre-element | 1 |
Entities:
Entity 1, miR-21 pre-element 29 residues - 111.103 Da.
| 1 | G | G | G | U | U | G | A | C | C | G | ||||
| 2 | U | U | G | A | A | U | C | U | C | A | ||||
| 3 | C | G | G | C | A | A | C | C | C |
Samples:
sample_1: miR-21 pre-element 1 mM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 273 K; pH: 6.7; pressure: 1 atm; ionic strength: 10 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz