BMRB Entry 19878
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19878
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Torres, Allan; Bansal, Paramjit; Koh, Jennifer; Pages, Guilhem; Wu, Ming; Kuchel, Philip. "Structure and antimicrobial activity of platypus 'intermediate' defensin-like peptide" FEBS Lett. 588, 1821-1826 (2014).
PubMed: 24694388
Assembly members:
Int-DLP, polymer, 44 residues, 5000.910 Da.
Natural source: Common Name: platypus Taxonomy ID: 9258 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ornithorhynchus anatinus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Int-DLP: RRRRRRPPCEDVNGQCQPRG
NPCLRLRGACPRGSRCCMPT
VAAH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 216 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Int-DLP | 1 |
Entities:
Entity 1, Int-DLP 44 residues - 5000.910 Da.
| 1 | ARG | ARG | ARG | ARG | ARG | ARG | PRO | PRO | CYS | GLU | ||||
| 2 | ASP | VAL | ASN | GLY | GLN | CYS | GLN | PRO | ARG | GLY | ||||
| 3 | ASN | PRO | CYS | LEU | ARG | LEU | ARG | GLY | ALA | CYS | ||||
| 4 | PRO | ARG | GLY | SER | ARG | CYS | CYS | MET | PRO | THR | ||||
| 5 | VAL | ALA | ALA | HIS |
Samples:
sample_1: Int-DLP1 – 2 mM
sample_conditions_1: temperature: 298 K; pH: 3.5; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, data analysis, structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz