BMRB Entry 19873

Name: D loop of tRNA(Met)
Published: 2015-04-13

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PDB ID: 2mn0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19873
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

D loop of tRNA(Met)

Deposition date:

2014-03-25

Original release date:

2015-04-13

Authors:

Eveline, Lescrinier; Natala, Dyubankova; Piet, Herdewijn

Citation:

Citation: Eveline, Lescrinier; Natalia, Dyubankova; Piet, Herdewijn. "Contribution of dihydrouridine in folding of the D-arm in tRNA" Org. Biomol. Chem. ., .-. (2015).
PubMed: 25815904

Assembly members:

Assembly members:
D_arm_tRNA(Met), polymer, 16 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D_arm_tRNA(Met): GGAGAGXGGAACUCCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	94
1H chemical shifts	110

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	D-arm of tRNA(Met)	1

Entities:

Entity 1, D-arm of tRNA(Met) 16 residues - Formula weight is not available

1

G

A

G

A

G

H2U

G

A

2

A

C

U

C

Samples:

sample_1: D arm tRNA(Met) 1 ± 0.5 mM; D2O 100%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - data analysis

X-PLOR_NIH v3.851, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz