BMRB Entry 19858

Name: NMR Studies of the Phosphorylation of the Mengovirus Leader Protein Reveal Stabilization of Intermolecular Domain Interactions
Published: 2014-10-07

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PDB ID: 2mml
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19858
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Studies of the Phosphorylation of the Mengovirus Leader Protein Reveal Stabilization of Intermolecular Domain Interactions

Deposition date:

2014-03-15

Original release date:

2014-10-07

Authors:

Bacot-Davis, Valjean; Porter, Frederick; Palmenberg, Ann

Citation:

Citation: Bacot-Davis, Valjean; Ciomperlik, J.; Basta, H.; Cornilescu, Claudia; Palmenberg, Ann. "Solution Structures of Mengovirus Leader Protein, its Phosphorylated Derivatives, and in Complex with Nuclear Transport Protein, RanGTPase" Proc. Nat. Acad. Sci, U.S.A. ., .-. (2014).

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, 8139.810 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: encephalomyocarditisvirus Taxonomy ID: 12107 Superkingdom: Viruses Kingdom: not available Genus/species: Cardiovirus Mengovirus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSTAMATTMEQEICAHSMTF EECPKCSALQYRNGFYLLKY DEEWYPEELLXDGEDDVFDP DLDMEVVFETQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	161
15N chemical shifts	69
1H chemical shifts	208

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION	2

Entities:

Entity 1, entity_1 71 residues - 8139.810 Da.

1

GLY

SER

THR

ALA

MET

ALA

THR

MET

GLU

2

GLN

GLU

ILE

CYS

ALA

HIS

SER

MET

THR

PHE

3

GLU

CYS

PRO

LYS

CYS

SER

ALA

LEU

GLN

4

TYR

ARG

ASN

GLY

PHE

TYR

LEU

LYS

TYR

5

ASP

GLU

TRP

TYR

PRO

GLU

LEU

6

TPO

ASP

GLY

GLU

ASP

VAL

PHE

ASP

PRO

7

ASP

LEU

ASP

MET

GLU

VAL

PHE

GLU

THR

8

GLN

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
31-P	sample_1	isotropic	sample_conditions_1

BMRB	19084 19855 19857
PDB	2M7Y 2MMH 2MMI 2MMK 2MML
EMBL	CAE84564 CAE84565 CAE84566
GB	AAA46547 AAB59755 ABB97066
SP	P12296 P32540
AlphaFold	P12296 P32540

BMRB Entry 19858

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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