BMRB Entry 19844

Name: 1H, 13C, and 15N backbone assignment of human ribonuclease 4
Published: 2014-04-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19844

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N backbone assignment of human ribonuclease 4

Deposition date:

2014-03-07

Original release date:

2014-04-07

Authors:

Gagne, Donald; Doucet, Nicolas

Citation:

Citation: Gagne, Donald; Doucet, Nicolas. "1H, 13C, and 15N backbone assignment of human ribonuclease 4" J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
RNase_4, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNase_4: QDGMYQRFLRQHVHPEETGG SDRYCNLMMQRRKMTLYHCK RFNTFIHEDIWNIRSICSTT NIQCKNGKMNCHEGVVKVTD CRDTGSSRAPNCRYRAIAST RRVVIACEGNPQVPVHFDG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	210
15N chemical shifts	110
1H chemical shifts	110

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNase 4	1

Entities:

Entity 1, RNase 4 119 residues - Formula weight is not available

1

GLN

ASP

GLY

MET

TYR

GLN

ARG

PHE

LEU

ARG

2

GLN

HIS

VAL

HIS

PRO

GLU

THR

GLY

3

SER

ASP

ARG

TYR

CYS

ASN

LEU

MET

GLN

4

ARG

LYS

MET

THR

LEU

TYR

HIS

CYS

LYS

5

ARG

PHE

ASN

THR

PHE

ILE

HIS

GLU

ASP

ILE

6

TRP

ASN

ILE

ARG

SER

ILE

CYS

SER

THR

7

ASN

ILE

GLN

CYS

LYS

ASN

GLY

LYS

MET

ASN

8

CYS

HIS

GLU

GLY

VAL

LYS

VAL

THR

ASP

9

CYS

ARG

ASP

THR

GLY

SER

ARG

ALA

PRO

10

ASN

CYS

ARG

TYR

ARG

ALA

ILE

ALA

SER

THR

11

ARG

VAL

ILE

ALA

CYS

GLU

GLY

ASN

12

PRO

GLN

VAL

PRO

VAL

HIS

PHE

ASP

GLY

Samples:

sample_1: RNase 4, [U-13C; U-15N], 0.56 mM; D2O 100%

sample_conditions_1: pH: 5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Agilent OMEGA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks