BMRB Entry 19828

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19828
MolProbity Validation Chart

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Title:
HUMAN CCR2 MEMBRANE-PROXIMAL C-TERMINAL REGION (PRO-C) IN A MEMBRANE BOUND FORM
Deposition date:
2014-03-04
Original release date:
2014-10-06
Authors:
Esaki, Kaori
Citation:

Citation: Esaki, K.. "Structural Insight Into the Interaction between the Membrane-Proximal C-Terminal Region of Chemokine Receptor Ccr2 and the Cytosolic Regulator Frount"  To be Published ., .-..

Assembly members:

Assembly members:
CHEMOKINE_(C-C_MOTIF)_RECEPTOR_2, polymer, 16 residues, 2133.564 Da.

Natural source:

Natural source:   Common Name: HUMAN   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: HOMO SAPIENS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PTKK19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CHEMOKINE_(C-C_MOTIF)_RECEPTOR_2: EKFRRYLSVFFRKHIT

Data sets:
Data typeCount
13C chemical shifts71
15N chemical shifts14
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CHEMOKINE (C-C MOTIF) RECEPTOR 21

Entities:

Entity 1, CHEMOKINE (C-C MOTIF) RECEPTOR 2 16 residues - 2133.564 Da.

1   GLULYSPHEARGARGTYRLEUSERVALPHE
2   PHEARGLYSHISILETHR

Samples:

CCR2_Pro-C_13C15N1: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; DPC, [U-99% 2H], 10 mM; D2O, [U-100% 2H], 100%

CCR2_Pro-C_13C15N2: CCR2_Pro-C, [U-100% 13C; U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; DPC, [U-99% 2H], 10 mM; D2O, [U-100% 2H], 5%; H2O 95%

CCR2_Pro-C_15N: CCR2_Pro-C, [U-100% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; DPC, [U-99% 2H], 10 mM; D2O, [U-100% 2H], 5%; H2O 95%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYCCR2_Pro-C_15Nisotropicsample_conditions_1
3D HNHACCR2_Pro-C_15Nisotropicsample_conditions_1
3D 1H- 13C NOESYCCR2_Pro-C_13C15N1isotropicsample_conditions_1
3D CBCA(CO)NHCCR2_Pro-C_13C15N2isotropicsample_conditions_1
3D HNCACBCCR2_Pro-C_13C15N2isotropicsample_conditions_1
3D HBHA(CO)NHCCR2_Pro-C_13C15N2isotropicsample_conditions_1
3D HCCH- TOCSYCCR2_Pro-C_13C15N1isotropicsample_conditions_1
2D 1H-15N HSQCCCR2_Pro-C_15Nisotropicsample_conditions_1
2D 1H-13C HSQCCCR2_Pro-C_13C15N1isotropicsample_conditions_1

Software:

CYANA, GUNTERT, MUMENTHALER - structure solution

NMR spectrometers:

  • BRUKER AVANCE III 600 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19829
PDB
DBJ BAA06253 BAD08648 BAD08655 BAD12134 BAF85374
EMBL CAA64835
GB AAA19120 AAB57792 AAC51636 AAH95540 ACN78985
REF NP_001001619 NP_001116868 XP_002832824 XP_003483213 XP_003926313

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