BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19825

Title: HLA-B*35:01 with peptide VPLRAMTY   PubMed: 25028510

Deposition date: 2014-03-02 Original release date: 2019-07-12

Authors: Yanaka, Saeko; Sugase, Kenji

Citation: Yanaka, Saeko; Ueno, Takamasa; Yi, Shi; Qi, Jianxun; Gao, George; Tsumoto, Kouhei; Sugase, Kenji. "Peptide-dependent conformational fluctuation determines the stability of the human leukocyte antigen class I complex"  J. Biol. Chem. 289, 24680-24690 (2014).

Assembly members:
HLA-B*35:01_with_peptide_VPLRAMTY, polymer, 276 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Entity Sequences (FASTA):
HLA-B*35:01_with_peptide_VPLRAMTY: GSHSMRYFYTAMSRPGRGEP RFIAVGYVDDTQFVRFDSDA ASPRTEPRPPWIEQEGPEYW DRNTQIFKTNTQTYRESLRN LRGYYNQSEAGSHIIQRMYG CDLGPDGRLLRGHDQSAYDG KDYIALNEDLSSWTAADTAA QITQRKWEAARVAEQLRAYL EGLCVEWLRRYLENGKETLQ RADPPKTHVTHHPVSDHEAT LRCWALGFYPAEITLTWQRD GEDQTQDTELVETRPAGDRT FQKWAAVVVPSGEEQRYTCH VQHEGLPKPLTLRWEP

Data sets:
Data typeCount
13C chemical shifts617
15N chemical shifts213
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta-2-microglobulin1

Entities:

Entity 1, beta-2-microglobulin 276 residues - Formula weight is not available

1   GLYSERHISSERMETARGTYRPHETYRTHR
2   ALAMETSERARGPROGLYARGGLYGLUPRO
3   ARGPHEILEALAVALGLYTYRVALASPASP
4   THRGLNPHEVALARGPHEASPSERASPALA
5   ALASERPROARGTHRGLUPROARGALAPRO
6   TRPILEGLUGLNGLUGLYPROGLUTYRTRP
7   ASPARGASNTHRGLNILEPHELYSTHRASN
8   THRGLNTHRTYRARGGLUSERLEUARGASN
9   LEUARGGLYTYRTYRASNGLNSERGLUALA
10   GLYSERHISILEILEGLNARGMETTYRGLY
11   CYSASPLEUGLYPROASPGLYARGLEULEU
12   ARGGLYHISASPGLNSERALATYRASPGLY
13   LYSASPTYRILEALALEUASNGLUASPLEU
14   SERSERTRPTHRALAALAASPTHRALAALA
15   GLNILETHRGLNARGLYSTRPGLUALAALA
16   ARGVALALAGLUGLNLEUARGALATYRLEU
17   GLUGLYLEUCYSVALGLUTRPLEUARGARG
18   TYRLEUGLUASNGLYLYSGLUTHRLEUGLN
19   ARGALAASPPROPROLYSTHRHISVALTHR
20   HISHISPROVALSERASPHISGLUALATHR
21   LEUARGCYSTRPALALEUGLYPHETYRPRO
22   ALAGLUILETHRLEUTHRTRPGLNARGASP
23   GLYGLUASPGLNTHRGLNASPTHRGLULEU
24   VALGLUTHRARGPROALAGLYASPARGTHR
25   PHEGLNLYSTRPALAALAVALVALVALPRO
26   SERGLYGLUGLUGLNARGTYRTHRCYSHIS
27   VALGLNHISGLUGLYLEUPROLYSPROLEU
28   THRLEUARGTRPGLUPRO

Samples:

sample_1: HLA-B*35:01 with peptide VPLRAMTY, [U-13C; U-15N; U-2H], 800 – 900 uM; potassium phosphate 20 mM; sodium chloride 143 mM; sodium azide 2 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts