BMRB Entry 19797

Name: STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD2b IN MICELLES
Published: 2014-05-12

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PDB ID: 2mkv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19797
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD2b IN MICELLES

Deposition date:

2014-02-13

Original release date:

2014-05-12

Authors:

Marassi, Francesca; Gong, Xiao-Min

Citation:

Citation: Gong, Xiao-Min; Marassi, Francesca. "Structure of the Na,K-ATPase regulatory protein FXYD2b in micelles: Implications for membrane-water interfacial arginines" Biochim. Biophys. Acta ., .-..
PubMed: 24794573

Assembly members:

Assembly members:
entity, polymer, 64 residues, 7331.390 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: LDRWYLGGSPKGDVDPFYYD YETVRNGGLIFAGLAFIVGL LILLSRRFRSGGNKKRRQIN EDEP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	169
15N chemical shifts	60
1H chemical shifts	229

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FXYD2b	1

Entities:

Entity 1, FXYD2b 64 residues - 7331.390 Da.

1

LEU

ASP

ARG

TRP

TYR

LEU

GLY

SER

PRO

2

LYS

GLY

ASP

VAL

ASP

PRO

PHE

TYR

ASP

3

TYR

GLU

THR

VAL

ARG

ASN

GLY

LEU

ILE

4

PHE

ALA

GLY

LEU

ALA

PHE

ILE

VAL

GLY

LEU

5

LEU

ILE

LEU

SER

ARG

PHE

ARG

SER

6

GLY

ASN

LYS

ARG

GLN

ILE

ASN

7

GLU

ASP

GLU

PRO

Samples:

sample_1: FXYD2b, [U-99% 13C; U-99% 15N], 0.2 mM; SDS micelles 500 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	anisotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2MKV
GB	AAG34359 AAG34361 AAG37906 AAH13289 AAX36673
REF	NP_001671
SP	P54710
AlphaFold	P54710