BMRB Entry 19779

Name: Solution structure of the SGTA N-terminal domain
Published: 2014-12-01

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PDB ID: 4cpg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19779
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SGTA N-terminal domain

Deposition date:

2014-02-07

Original release date:

2014-12-01

Authors:

Darby, J.; Krysztofinska, E.; Simpson, P.; Isaacson, Rivka

Citation:

Citation: Darby, J.; Krysztofinska, E.; Simpson, P.; Simon, A.; Sriskandarajah, N.; Bishop, D.; Hale, L.; Alfano, C.; Conte, M.; High, S.; Isaacson, Rivka. "Solution structure of the SGTA dimerisation domain and investigation of its interactions with the ubiquitin-like domains of BAG6 and UBL4A" PLOS ONE ., .-..

Assembly members:

Assembly members:
SMALL_GLUTAMINE-RICH_TETRATRICOPEPTIDE_REPEAT-CON_PROTEIN_ALPHA, polymer, 83 residues, 9228.2247 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SMALL_GLUTAMINE-RICH_TETRATRICOPEPTIDE_REPEAT-CON_PROTEIN_ALPHA: MAHHHHHHVDDDDKMDNKKR LAYAIIQFLHDQLRHGGLSS DAQESLEVAIQCLETAFGVT VEDSDLALPQTLPEIFEAAA TGK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	303
15N chemical shifts	73
1H chemical shifts	485

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, 1	1
2	SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, 2	1

Entities:

Entity 1, SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, 1 83 residues - 9228.2247 Da.

1

MET

ALA

HIS

VAL

ASP

2

ASP

LYS

MET

ASP

ASN

LYS

ARG

3

LEU

ALA

TYR

ALA

ILE

GLN

PHE

LEU

HIS

4

ASP

GLN

LEU

ARG

HIS

GLY

LEU

SER

5

ASP

ALA

GLN

GLU

SER

LEU

GLU

VAL

ALA

ILE

6

GLN

CYS

LEU

GLU

THR

ALA

PHE

GLY

VAL

THR

7

VAL

GLU

ASP

SER

ASP

LEU

ALA

LEU

PRO

GLN

8

THR

LEU

PRO

GLU

ILE

PHE

GLU

ALA

9

THR

GLY

LYS

Samples:

sample_1: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, [U-13C; U-15N], 0.75 mM

sample_2: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, [U-13C; U-15N], 0.75 mM

sample_3: SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA, [U-13C; U-15N], 0.4 mM; SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CON PROTEIN ALPHA 0.4 mM

sample_conditions_1: ionic strength: 250.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 303.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	solution	sample_conditions_1
3D HNCACB	sample_1	solution	sample_conditions_1
3D CBCA(CO)NH	sample_1	solution	sample_conditions_1
3D HNCO	sample_1	solution	sample_conditions_1
3D HN(CA)CO	sample_1	solution	sample_conditions_1
3D HBHA(CO)NH	sample_1	solution	sample_conditions_1
3D H(CCCO)NH	sample_1	solution	sample_conditions_1
3D (H)CCCONH	sample_1	solution	sample_conditions_1
3D 1H-15N NOESY	sample_1	solution	sample_conditions_1
3D HCCH-TOCSY	sample_2	solution	sample_conditions_1
3D (H)CCH-TOCSY	sample_2	solution	sample_conditions_1
3D 1H-13C NOESY	sample_2	solution	sample_conditions_1
3D F1 1H(13C	sample_3	solution	sample_conditions_1
15N)-reject 13C NOESY	sample_3	solution	sample_conditions_1

Software:

ARIA vany, Linge, O'Donoghue and Nilges - chemical shift assignment

AutoDep v4.3, pDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMRView vany, Johnson, One Moon Scientific - chemical shift assignment

TALOS vany, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Other Other 950 MHz
Bruker Avance 800 MHz
Bruker Avance 700 MHz

UNP	SGTA_HUMAN
PDB	4CPG 4GOD 4GOE 4GOF
DBJ	BAE01321 BAG57017 BAG73729
EMBL	CAA11565 CAB39725 CAB43297 CAG38548 CAG47077
GB	AAD13117 AAH00390 AAH02989 AAH05165 AAH08885
PIR	T08782
REF	NP_001181661 NP_003012 XP_001914923 XP_002761612 XP_003277059
SP	O43765
AlphaFold	Q9BTZ9 O43765