BMRB Entry 19773

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19773
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
Backbone chemical shifts for the E81 deletion mutant from RAP80 tandem UIMs
Deposition date:
2014-02-06
Original release date:
2014-02-06
Authors:
Anamika, Anamika; Markin, Craig; Rout, Manoj; Spyracopoulos, Leo
Citation:

Citation: Anamika, Anamika; Markin, Craig; Rout, Manoj; Spyracopoulos, Leo. "Molecular Basis for Impaired DNA Damage Response Function Associated with the RAP80 E81 Defect."  J. Biol. Chem. ., .-. (2014).
PubMed: 24627472

Assembly members:

Assembly members:
entity, polymer, 62 residues, 6889.636 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPLGSRKIAQMTEEQFALAL KMSEQEAREVNSQEEEEEEL LRKAIAESLNSCRPSDASAT RS

Data sets:
Data typeCount
13C chemical shifts113
15N chemical shifts58
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 62 residues - 6889.636 Da.

1   GLYPROLEUGLYSERARGLYSILEALAGLN
2   METTHRGLUGLUGLNPHEALALEUALALEU
3   LYSMETSERGLUGLNGLUALAARGGLUVAL
4   ASNSERGLNGLUGLUGLUGLUGLUGLULEU
5   LEUARGLYSALAILEALAGLUSERLEUASN
6   SERCYSARGPROSERASPALASERALATHR
7   ARGSER

Samples:

sample_1: deltaE81-RAP80-tUIM, [U-100% 15N], .5 – 1 mM; TRIS 50 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O 10%

sample_2: deltaE81-RAP80-tUIM, [U-100% 13C; U-100% 15N], .5 – 1 mM; TRIS 50 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K

sample_conditions_2: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HBHA(CO)NHsample_2isotropicsample_conditions_2

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 19774
PDB
GB AAG59851 ERE80156
REF XP_006220429 XP_007124893 XP_008013593 XP_008988510 XP_012516199

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks