BMRB Entry 19767
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19767
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Citation: Banci, Lucia; Blaevit, Olga; Cantini, Francesca; Danielsson, Jens; Lang, Lisa; Luchinat, Claudio; Mao, Jiafei; Oliveberg, Mikael; Ravera, Enrico. "Solid-state NMR studies of metal-free SOD1 fibrillar structures." J. Biol. Inorg. Chem. 19, 659-666 (2014).
PubMed: 24719206
Assembly members:
apoSOD1-DeltaIV-VII, polymer, 110 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
apoSOD1-DeltaIV-VII: ATKAVAVLKGDGPVQGIINF
EQKESNGPVKVWGSIKGLTE
GLHGFHVHGAGGDLGNVTAD
KDGVADVSIEDSVISLSGDH
SIIGRTLVVHEKAGAGAGSR
LASGVIGIAQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 79 |
| 15N chemical shifts | 28 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoSOD1-DeltaIV-VII | 1 |
Entities:
Entity 1, apoSOD1-DeltaIV-VII 110 residues - Formula weight is not available
| 1 | ALA | THR | LYS | ALA | VAL | ALA | VAL | LEU | LYS | GLY | |
| 2 | ASP | GLY | PRO | VAL | GLN | GLY | ILE | ILE | ASN | PHE | |
| 3 | GLU | GLN | LYS | GLU | SER | ASN | GLY | PRO | VAL | LYS | |
| 4 | VAL | TRP | GLY | SER | ILE | LYS | GLY | LEU | THR | GLU | |
| 5 | GLY | LEU | HIS | GLY | PHE | HIS | VAL | HIS | GLY | ALA | |
| 6 | GLY | GLY | ASP | LEU | GLY | ASN | VAL | THR | ALA | ASP | |
| 7 | LYS | ASP | GLY | VAL | ALA | ASP | VAL | SER | ILE | GLU | |
| 8 | ASP | SER | VAL | ILE | SER | LEU | SER | GLY | ASP | HIS | |
| 9 | SER | ILE | ILE | GLY | ARG | THR | LEU | VAL | VAL | HIS | |
| 10 | GLU | LYS | ALA | GLY | ALA | GLY | ALA | GLY | SER | ARG | |
| 11 | LEU | ALA | SER | GLY | VAL | ILE | GLY | ILE | ALA | GLN |
Samples:
sample_1: apoSOD1-DeltaIV-VII, [U-13C; U-15N], na na
sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DARR | sample_1 | solid | sample_conditions_1 |
| 2D NCA | sample_1 | solid | sample_conditions_1 |
| 2D NCO | sample_1 | solid | sample_conditions_1 |
| 3D NCACX | sample_1 | solid | sample_conditions_1 |
| 3D NCOCX | sample_1 | solid | sample_conditions_1 |
| 1H-13C INEPT-HETCOR | sample_1 | solid | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 850 MHz
- Bruker Avance 700 MHz
Related Database Links:
| PDB |