BMRB Entry 19754

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19754

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Title:
Chemical shifts assignments of diacylglycerol kinase (DAGK) by solid-state NMR
Deposition date:
2014-01-27
Original release date:
2014-01-27
Authors:
Chen, Yanke; Zhang, Zhengfeng; Tang, Xinqi; Li, Jianping; Yang, Jun; Glaubitz, Clemens
Citation:

Citation: Chen, Yanke; Zhang, Zhengfeng; Tang, Xinqi; Li, Jianping; Glaubitz, Clemens; Yang, Jun. "Conformation and Topology of Diacylglycerol Kinase in E.coli Membranes Revealed by Solid-state NMR Spectroscopy."  Angew. Chem. Int. Ed. Engl. ., .-. (2014).
PubMed: 24700682

Assembly members:

Assembly members:
DAGK, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSD005

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DAGK: MGHHHHHHELANNTTGFTRI IKAAGYSWKGLRAAWINEAA FRQEGVAVLLAVVIACWLDV DACTRVLLISSVMLVMIVEL LNSAIEAVVDRIGSEYHELS GRAKDMGSAAVLIAIIDAVI TWCILLWSHF

Data sets:
Data typeCount
13C chemical shifts369
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DAGK1

Entities:

Entity 1, DAGK 130 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISGLULEU
2   ALAASNASNTHRTHRGLYPHETHRARGILE
3   ILELYSALAALAGLYTYRSERTRPLYSGLY
4   LEUARGALAALATRPILEASNGLUALAALA
5   PHEARGGLNGLUGLYVALALAVALLEULEU
6   ALAVALVALILEALACYSTRPLEUASPVAL
7   ASPALACYSTHRARGVALLEULEUILESER
8   SERVALMETLEUVALMETILEVALGLULEU
9   LEUASNSERALAILEGLUALAVALVALASP
10   ARGILEGLYSERGLUTYRHISGLULEUSER
11   GLYARGALALYSASPMETGLYSERALAALA
12   VALLEUILEALAILEILEASPALAVALILE
13   THRTRPCYSILELEULEUTRPSERHISPHE

Samples:

sample_1: DGAK, [U-13C; U-15N],

sample_2: DGAK, [U-13C; U-15N]; [U-12C; U-14N]-Leu,Ile,

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
3D CONCAsample_1solidsample_conditions_1
3D CAN(CO)CXsample_1solidsample_conditions_1
3D CON(CA)CXsample_1solidsample_conditions_1
3D CONCAsample_2solidsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

VNMRJ v2.31A, Varian - collection

NMR spectrometers:

  • Varian Uniform NMR System 600 MHz

Related Database Links:

PDB
GB EKW72221 ETJ14673