BMRB Entry 19737

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19737
MolProbity Validation Chart

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Title:
Solution structure of a C terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB)
Deposition date:
2014-01-16
Original release date:
2014-02-04
Authors:
Esteve, Vicent; Blatter, Markus; Allain, Frederic
Citation:

Citation: Joshi, Amar; Esteve, Vicent; Buckroyd, Adrian; Blatter, Markus; Allain, Frederic H-T; Curry, Stephen. "Solution and crystal structures of a C-terminal fragment of the neuronal isoform of the polypyrimidine tract binding protein (nPTB)."  PeerJ 2, e305-e305 (2014).
PubMed: 24688880

Assembly members:

Assembly members:
nPTB, polymer, 207 residues, 22919.373 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nPTB: GRVGMPGVSAGGNTVLLVSN LNEEMVTPQSLFTLFGVYGD VQRVKILYNKKDSALIQMAD GNQSQLAMNHLNGQKMYGKI IRVTLSKHQTVQLPREGLDD QGLTKDFGNSPLHRFKKPGS KNFQNIFPPSATLHLSNIPP SVAEEDLRTLFANTGGTVKA FKFFQDHKMALLQMATVEEA IQALIDLHNYNLGENHHLRV SFSKSTI

Data sets:
Data typeCount
13C chemical shifts472
15N chemical shifts194
1H chemical shifts1103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C terminal fragment of nPTB1

Entities:

Entity 1, C terminal fragment of nPTB 207 residues - 22919.373 Da.

1   GLYARGVALGLYMETPROGLYVALSERALA
2   GLYGLYASNTHRVALLEULEUVALSERASN
3   LEUASNGLUGLUMETVALTHRPROGLNSER
4   LEUPHETHRLEUPHEGLYVALTYRGLYASP
5   VALGLNARGVALLYSILELEUTYRASNLYS
6   LYSASPSERALALEUILEGLNMETALAASP
7   GLYASNGLNSERGLNLEUALAMETASNHIS
8   LEUASNGLYGLNLYSMETTYRGLYLYSILE
9   ILEARGVALTHRLEUSERLYSHISGLNTHR
10   VALGLNLEUPROARGGLUGLYLEUASPASP
11   GLNGLYLEUTHRLYSASPPHEGLYASNSER
12   PROLEUHISARGPHELYSLYSPROGLYSER
13   LYSASNPHEGLNASNILEPHEPROPROSER
14   ALATHRLEUHISLEUSERASNILEPROPRO
15   SERVALALAGLUGLUASPLEUARGTHRLEU
16   PHEALAASNTHRGLYGLYTHRVALLYSALA
17   PHELYSPHEPHEGLNASPHISLYSMETALA
18   LEULEUGLNMETALATHRVALGLUGLUALA
19   ILEGLNALALEUILEASPLEUHISASNTYR
20   ASNLEUGLYGLUASNHISHISLEUARGVAL
21   SERPHESERLYSSERTHRILE

Samples:

sample_1: nPTB, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 20 mM; sodium phosphate 10 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: nPTB, [U-100% 15N], 1 mM; sodium chloride 20 mM; sodium phosphate 10 mM; D2O, [U-100% 2H], 100%

sample_conditions_1: ionic strength: 0.03 M; pH: 5.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

AMBER v12, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - nOe assignment, structure calculation

TOPSPIN v3.1, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAB28977 BAB71742 BAE23317 BAE89548 BAG61644
EMBL CAB54073
GB AAF14284 AAF21807 AAH10255 AAH16582 AAH82076
PIR JC7526
REF NP_001005555 NP_001287914 NP_001287915 NP_001287916 NP_001287917
SP Q66H20 Q91Z31 Q9UKA9
TPG DAA00060 DAA00061 DAA31417
AlphaFold Q66H20 Q91Z31 Q9UKA9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks