Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19719
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Citation: Oberli, Alexander; Slater, Leanne; Cutts, Erin; Brand, Francoise; Mundwiler-Pachlatko, Esther; Rusch, Sebastian; Masik, Martin; Erat, Michele; Beck, Hans-Peter; Vakonakis, Ioannis. "A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 and comigrates to knobs on the host cell surface" FASEB J. 28, 4420-4433 (2014).
PubMed: 24983468
Assembly members:
PFI1780w, polymer, 155 residues, Formula weight is not available
Natural source: Common Name: malaria parasite P. falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-2
Data type | Count |
13C chemical shifts | 419 |
15N chemical shifts | 148 |
1H chemical shifts | 414 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PHIST | 1 |
Entity 1, PHIST 155 residues - Formula weight is not available
Residues 1-5 represent a cloning artefact
1 | GLY | PRO | LEU | GLY | SER | TYR | THR | ALA | GLU | GLU | ||||
2 | ILE | ASN | GLU | MET | ILE | ASN | SER | SER | ASN | GLU | ||||
3 | PHE | ILE | ASN | ARG | ASN | ASP | MET | ASN | ILE | ILE | ||||
4 | PHE | SER | TYR | VAL | HIS | GLU | SER | GLU | ARG | GLU | ||||
5 | LYS | PHE | LYS | LYS | VAL | GLU | GLU | ASN | ILE | PHE | ||||
6 | LYS | PHE | ILE | GLN | SER | ILE | VAL | GLU | THR | TYR | ||||
7 | LYS | ILE | PRO | ASP | GLU | TYR | LYS | MET | ARG | LYS | ||||
8 | PHE | LYS | PHE | ALA | HIS | PHE | GLU | MET | GLN | GLY | ||||
9 | TYR | ALA | LEU | LYS | GLN | GLU | LYS | PHE | LEU | LEU | ||||
10 | GLU | TYR | ALA | PHE | LEU | SER | LEU | ASN | GLY | LYS | ||||
11 | LEU | CYS | GLU | ARG | LYS | LYS | PHE | LYS | GLU | VAL | ||||
12 | LEU | GLU | TYR | VAL | LYS | ARG | GLU | TRP | ILE | GLU | ||||
13 | PHE | ARG | LYS | SER | MET | PHE | ASP | VAL | TRP | LYS | ||||
14 | GLU | LYS | LEU | ALA | SER | GLU | PHE | ARG | GLU | HIS | ||||
15 | GLY | GLU | MET | LEU | ASN | GLN | LYS | ARG | LYS | LEU | ||||
16 | LYS | GLN | HIS | GLU | LEU |
sample_1: PFI1780w, [U-99% 13C; U-99% 15N], 0.2 mM; D2O 5 % v/v; H2O 95 % v/v; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 0.02 % w/v; DTT 2 mM; DSS 0.1 mM
sample_conditions_1: ionic strength: 110 mM; pH: 7; pressure: 1 atm; temperature: 310.15 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PIPP, Garrett - data analysis, peak picking
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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or all simulated peaks