BMRB Entry 19711

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19711

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Title:
Backbone and side chain 1H, 13C, and 15N chemical Shift assignments for native split Npu DnaE intein
Deposition date:
2013-12-30
Original release date:
2014-06-06
Authors:
Lee, Yi-Zong; Sue, Shih-Che
Citation:

Citation: Lee, Yi-Zong; Lee, Yun-Tzai; Lin, Yi-Jan; Chen, Yen-Ju; Sue, Shih-Che. "A streamlined method for preparing split intein for NMR study."  Protein Expr. Purif. 99, 106-112 (2014).
PubMed: 24751877

Assembly members:

Assembly members:
Native_split_Npu_DnaE_intein, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-28A(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Native_split_Npu_DnaE_intein: CLSYETEILTVEYGLLPIGK IVEKRIECTVYSVDNNGNIY TQPVAQWHDRGEQEVFEYCL EDGSLIRATKDHKFMTVDGQ MLPIDEIFERELDLMRVDNL PNAAALEHHHHHHMGIKIAT RKYLGKQNVYDIGVERDHNF ALKNGFIASN

Data sets:
Data typeCount
13C chemical shifts536
15N chemical shifts136
1H chemical shifts865

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1native split Npu DnaE intein1

Entities:

Entity 1, native split Npu DnaE intein 150 residues - Formula weight is not available

N fragment with residue 1-102 and a C-terminal his tag C fragment with residue 103-137

1   CYSLEUSERTYRGLUTHRGLUILELEUTHR
2   VALGLUTYRGLYLEULEUPROILEGLYLYS
3   ILEVALGLULYSARGILEGLUCYSTHRVAL
4   TYRSERVALASPASNASNGLYASNILETYR
5   THRGLNPROVALALAGLNTRPHISASPARG
6   GLYGLUGLNGLUVALPHEGLUTYRCYSLEU
7   GLUASPGLYSERLEUILEARGALATHRLYS
8   ASPHISLYSPHEMETTHRVALASPGLYGLN
9   METLEUPROILEASPGLUILEPHEGLUARG
10   GLULEUASPLEUMETARGVALASPASNLEU
11   PROASNALAALAALALEUGLUHISHISHIS
12   HISHISHISMETGLYILELYSILEALATHR
13   ARGLYSTYRLEUGLYLYSGLNASNVALTYR
14   ASPILEGLYVALGLUARGASPHISASNPHE
15   ALALEULYSASNGLYPHEILEALASERASN

Samples:

sample_NATIVE_split_Npu_DnaE_intein: Native split Npu DnaE intein, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HNCOsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HNCAsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HNCACBsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HBHA(CO)NHsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HN(CO)CAsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HN(COCA)CBsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D H(CCO)NHsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HCCH-TOCSYsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D HCCH-COSYsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D 1H-15N NOESYsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_NATIVE_split_Npu_DnaE_inteinisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
GB ACC83218 ADG44968 ADG44969 AIS22787 AIS22800
REF WP_012411174

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks