BMRB Entry 19681
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PDB ID: 2mii
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19681
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Jean, Nicolas; Bougault, Catherine; Egan, Alexander; Vollmer, Waldemar; Simorre, Jean-Pierre. "Solution NMR assignment of LpoB, an outer-membrane anchored Penicillin-Binding Protein activator from Escherichia coli." Biomol. NMR Assignments ., .-. (2014).
PubMed: 24691651
Assembly members:
LpoB, polymer, 197 residues, 20770.459 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28LpoB
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 756 |
| 15N chemical shifts | 197 |
| 1H chemical shifts | 1165 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E. coli LpoB | 1 |
Entities:
Entity 1, E. coli LpoB 197 residues - 20770.459 Da.
Residues 1 to 4 are reminiscent of the hexahistidine-tag left after thrombine cleavage
| 1 | GLY | SER | HIS | MET | VAL | GLY | GLN | ARG | GLU | PRO | ||||
| 2 | ALA | PRO | VAL | GLU | GLU | VAL | LYS | PRO | ALA | PRO | ||||
| 3 | GLU | GLN | PRO | ALA | GLU | PRO | GLN | GLN | PRO | VAL | ||||
| 4 | PRO | THR | VAL | PRO | SER | VAL | PRO | THR | ILE | PRO | ||||
| 5 | GLN | GLN | PRO | GLY | PRO | ILE | GLU | HIS | GLU | ASP | ||||
| 6 | GLN | THR | ALA | PRO | PRO | ALA | PRO | HIS | ILE | ARG | ||||
| 7 | HIS | TYR | ASP | TRP | ASN | GLY | ALA | MET | GLN | PRO | ||||
| 8 | MET | VAL | SER | LYS | MET | LEU | GLY | ALA | ASP | GLY | ||||
| 9 | VAL | THR | ALA | GLY | SER | VAL | LEU | LEU | VAL | ASP | ||||
| 10 | SER | VAL | ASN | ASN | ARG | THR | ASN | GLY | SER | LEU | ||||
| 11 | ASN | ALA | ALA | GLU | ALA | THR | GLU | THR | LEU | ARG | ||||
| 12 | ASN | ALA | LEU | ALA | ASN | ASN | GLY | LYS | PHE | THR | ||||
| 13 | LEU | VAL | SER | ALA | GLN | GLN | LEU | SER | MET | ALA | ||||
| 14 | LYS | GLN | GLN | LEU | GLY | LEU | SER | PRO | GLN | ASP | ||||
| 15 | SER | LEU | GLY | THR | ARG | SER | LYS | ALA | ILE | GLY | ||||
| 16 | ILE | ALA | ARG | ASN | VAL | GLY | ALA | HIS | TYR | VAL | ||||
| 17 | LEU | TYR | SER | SER | ALA | SER | GLY | ASN | VAL | ASN | ||||
| 18 | ALA | PRO | THR | LEU | GLN | MET | GLN | LEU | MET | LEU | ||||
| 19 | VAL | GLN | THR | GLY | GLU | ILE | ILE | TRP | SER | GLY | ||||
| 20 | LYS | GLY | ALA | VAL | SER | GLN | GLN |
Related Database Links:
| UNP | P0AB38 |
| PDB | 2MII 4Q6Z |
| DBJ | BAA35912 BAB34906 BAG76694 BAI24739 BAI29989 |
| EMBL | CAP75597 CAQ31626 CAQ98004 CAR02445 CAR07449 |
| GB | AAC74189 AAG55851 AAN79848 AAP16615 AAZ87850 |
| REF | NP_309510 NP_415623 WP_000164436 WP_000164438 WP_000164439 |
| SP | P0AB38 P0AB39 Q3Z312 |
| AlphaFold | Q9R424 P0AB38 P0AB39 Q3Z312 |
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