BMRB Entry 19667

Name: Solution structure of the carboxyterminal domain of NusG from Mycobacterium tuberculosis
Published: 2014-01-27

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PDB ID: 2mi6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19667
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the carboxyterminal domain of NusG from Mycobacterium tuberculosis

Deposition date:

2013-12-09

Original release date:

2014-01-27

Authors:

Strauss, Martin; Schweimer, Kristian; Roesch, Paul

Citation:

Citation: Strauss, Martin; Schweimer, Kristian; Roesch, Paul. "Solution structure of the carboxyterminal domain of NusG from mycobacterium tuberculosis" .

Assembly members:

Assembly members:
entity, polymer, 62 residues, 6711.688 Da.

Natural source:

Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET101/D-TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GRPVVEVDYEVGESVTVMDG PFATLPATISEVNAEQQKLK VLVSIFGRETPVELTFGQVS KI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	263
15N chemical shifts	60
1H chemical shifts	446

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	carboxyterminal domain of NusG	1

Entities:

Entity 1, carboxyterminal domain of NusG 62 residues - 6711.688 Da.

1

GLY

ARG

PRO

VAL

GLU

VAL

ASP

TYR

GLU

2

VAL

GLY

GLU

SER

VAL

THR

VAL

MET

ASP

GLY

3

PRO

PHE

ALA

THR

LEU

PRO

ALA

THR

ILE

SER

4

GLU

VAL

ASN

ALA

GLU

GLN

LYS

LEU

LYS

5

VAL

LEU

VAL

SER

ILE

PHE

GLY

ARG

GLU

THR

6

PRO

VAL

GLU

LEU

THR

PHE

GLY

GLN

VAL

SER

7

LYS

ILE

Samples:

sample_1: entity, [U-95% 13C; U-90% 15N], 1.1 mM; HEPES 25 mM; sodium chloride 150 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView, Johnson, One Moon Scientific - NMR data analysis

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2MI6
DBJ	BAH24951 BAL64520 BAQ04534 GAA44419
EMBL	CAL70674 CCC25720 CCC42978 CCC63246 CCE36182
GB	AAK44893 ABQ72374 ABR04995 ACT23685 AEB02783
REF	NP_215153 NP_854316 WP_003403288 WP_023349453 WP_023370810
SP	P65590 P9WIU8 P9WIU9
AlphaFold	P65590 P9WIU8 P9WIU9