BMRB Entry 19656

Name: CD79b cytosolic domain phosphorylated, denatured state
Published: 2014-02-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19656

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CD79b cytosolic domain phosphorylated, denatured state

Deposition date:

2013-12-04

Original release date:

2014-02-11

Authors:

Rosenlow, Joakim

Citation:

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Rosenlow, Joakim; Brutscher, Bernhard; Karlsson, B. Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains." PLoS ONE 8, e62947-e62947 (2013).
PubMed: 23667548

Assembly members:

Assembly members:
CD79b_cyt_phosp, polymer, 51 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: pEXP5-NT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD79b_cyt_phosp: SLLDKDDSKAGMEEDHTXEG LDIDQTATXEDIVTLRTGEV KWSVGEHPGQE

Data sets:

assigned_chemical_shifts
- CD79b_cyt_phosph_denatured_assigned_chem_shift_list

Data type	Count
13C chemical shifts	138
15N chemical shifts	47
1H chemical shifts	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD79b_cyt_phosp	1

Entities:

Entity 1, CD79b_cyt_phosp 51 residues - Formula weight is not available

1

SER

LEU

ASP

LYS

ASP

SER

LYS

ALA

2

GLY

MET

GLU

ASP

HIS

THR

PTR

GLU

GLY

3

LEU

ASP

ILE

ASP

GLN

THR

ALA

THR

PTR

GLU

4

ASP

ILE

VAL

THR

LEU

ARG

THR

GLY

GLU

VAL

5

LYS

TRP

SER

VAL

GLY

GLU

HIS

PRO

GLY

GLN

6

GLU

Samples:

CD79b_cytosolic_domain_phosphorylated_denatured: CD79b_cyt_phosp, [U-99% 13C; U-99% 15N], 200 – 300 uM; DTT 2 mM; sodium phosphate 20 mM; sodium azide 0.02%; Complete EDTA-free 1 X; TSP 100 uM; magnesium chloride 12 mM; ATP 1 mM; Fyn kinase 300 nM; sodium chloride 150 mM; urea 6 M; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.000; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1
3D HN(ca)CO	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1
3D HNCA	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1
3D HNCACB	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1
3D HNCO	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1
3D HN(CO)CA	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1
3D HN(COCA)CB	CD79b_cytosolic_domain_phosphorylated_denatured	isotropic	sample_conditions_1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - Spectrum analysis

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing

TANSY v1.0, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

Varian Unity Inova 600 MHz

UniProt	P40259
BMRB	19655
AlphaFold	Q9BU06