BMRB Entry 19637

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PDB ID: 2mhl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19637
MolProbity Validation Chart

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Title:
NMR solution Structure of the E.coli Outer Membrane Protein W
Deposition date:
2013-11-26
Original release date:
2014-08-04
Authors:
Horst, R.; Stanczak, P.; Wuthrich, K
Citation:

Citation: Horst, Reto; Stanczak, Pawel; Wuthrich, Kurt. "NMR polypeptide backbone conformation of the E. coli outer membrane protein W."  Structure 22, 1204-1209 (2014).
PubMed: 25017731

Assembly members:

Assembly members:
Outer_Membrane_Protein_W, polymer, 192 residues, 21002.574 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Outer_Membrane_Protein_W: MHEAGEFFMRAGSATVRPTE GAGGTLGSLGGFSVTNNTQL GLTFTYMATDNIGVELLAAT PFRHKIGTRATGDIATVHHL PPTLMAQWYFGDASSKFRPY VGAGINYTTFFDNGFNDHGK EAGLSDLSLKDSWGAAGQVG VDYLINRDWLVNMSVWYMDI DTTANYKLGGAQQHDSVRLD PWVFMFSAGYRF

Data sets:
Data typeCount
1H chemical shifts177
13C chemical shifts470
15N chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Outer Membrane Protein X from E. coli1

Entities:

Entity 1, Outer Membrane Protein X from E. coli 192 residues - 21002.574 Da.

1   METHISGLUALAGLYGLUPHEPHEMETARG
2   ALAGLYSERALATHRVALARGPROTHRGLU
3   GLYALAGLYGLYTHRLEUGLYSERLEUGLY
4   GLYPHESERVALTHRASNASNTHRGLNLEU
5   GLYLEUTHRPHETHRTYRMETALATHRASP
6   ASNILEGLYVALGLULEULEUALAALATHR
7   PROPHEARGHISLYSILEGLYTHRARGALA
8   THRGLYASPILEALATHRVALHISHISLEU
9   PROPROTHRLEUMETALAGLNTRPTYRPHE
10   GLYASPALASERSERLYSPHEARGPROTYR
11   VALGLYALAGLYILEASNTYRTHRTHRPHE
12   PHEASPASNGLYPHEASNASPHISGLYLYS
13   GLUALAGLYLEUSERASPLEUSERLEULYS
14   ASPSERTRPGLYALAALAGLYGLNVALGLY
15   VALASPTYRLEUILEASNARGASPTRPLEU
16   VALASNMETSERVALTRPTYRMETASPILE
17   ASPTHRTHRALAASNTYRLYSLEUGLYGLY
18   ALAGLNGLNHISASPSERVALARGLEUASP
19   PROTRPVALPHEMETPHESERALAGLYTYR
20   ARGPHE

Related Database Links:

PDB 2F1T 2F1V 2MHL
DBJ BAA14788 BAG66417 BAG76829 BAJ43052 BAL38320
EMBL CAA31922 CAP75801 CAQ31757 CAQ98135 CAR12762
GB AAB60065 AAB60073 AAB60081 AAB60089 AAB60097
REF NP_415772 NP_707165 WP_000737220 WP_000737221 WP_000737222
SP P0A915 P0A916
AlphaFold P0A915 P0A916

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks