BMRB Entry 19626

Name: 1H,13C and 15N assignment of Rsa1p(317-352)/Hit1p((70-164)
Published: 2014-09-08

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PDB ID: 2mjf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19626
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,13C and 15N assignment of Rsa1p(317-352)/Hit1p((70-164)

Deposition date:

2013-11-21

Original release date:

2014-09-08

Authors:

Quinternet, Marc; Roth, Benjamin; Back, Regis; Jacquemin, Clemence; Manival, Xavier

Citation:

Citation: Rothe, Benjamin; Saliou, Jean-Michel; Quinternet, Marc; Back, Regis; Tiotiu, Decebal; Jacquemin, Clemence; Loegler, Christine; Schlotter, Florence; Pena, Vlad; Eckert, Kelvin; Morera, Solange; Dorsselaer, Alain Van; Branlant, Christiane; Massenet, Severine; Sanglier-Cianferani, Sarah; Manival, Xavier; Charpentier, Bruno. "Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction." Nucleic Acids Res. 42, 10731-10747 (2014).
PubMed: 25170085

Assembly members:

Assembly members:
rsa, polymer, 63 residues, Formula weight is not available
hit, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pnEA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rsa: GPHMFANENSQLLDFIRELG DVGLLEYELSQQEKDVLFGS SEDNNKNHYKPNYKNRKPNL SRA
hit: MSEQPRAGTEANVEVVNNDK IINSSLAMNKTLKTKAFDDI YQNSAELQELLKYNTVKFHL AKVYRILSSTVNDGSSGKMN SDLQKELAVNYLNTLRYGGI HYNEAIEEFCQILLDKLNAV KK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	596
15N chemical shifts	149
1H chemical shifts	970

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rsa	1
2	hit	2

Entities:

Entity 1, rsa 63 residues - Formula weight is not available

1

GLY

PRO

HIS

MET

PHE

ALA

ASN

GLU

ASN

SER

2

GLN

LEU

ASP

PHE

ILE

ARG

GLU

LEU

GLY

3

ASP

VAL

GLY

LEU

GLU

TYR

GLU

LEU

SER

4

GLN

GLU

LYS

ASP

VAL

LEU

PHE

GLY

SER

5

SER

GLU

ASP

ASN

LYS

ASN

HIS

TYR

LYS

6

PRO

ASN

TYR

LYS

ASN

ARG

LYS

PRO

ASN

LEU

7

SER

ARG

ALA

Entity 2, hit 122 residues - Formula weight is not available

1

MET

SER

GLU

GLN

PRO

ARG

ALA

GLY

THR

GLU

2

ALA

ASN

VAL

GLU

VAL

ASN

ASP

LYS

3

ILE

ASN

SER

LEU

ALA

MET

ASN

LYS

4

THR

LEU

LYS

THR

LYS

ALA

PHE

ASP

ILE

5

TYR

GLN

ASN

SER

ALA

GLU

LEU

GLN

GLU

LEU

6

LEU

LYS

TYR

ASN

THR

VAL

LYS

PHE

HIS

LEU

7

ALA

LYS

VAL

TYR

ARG

ILE

LEU

SER

THR

8

VAL

ASN

ASP

GLY

SER

GLY

LYS

MET

ASN

9

SER

ASP

LEU

GLN

LYS

GLU

LEU

ALA

VAL

ASN

10

TYR

LEU

ASN

THR

LEU

ARG

TYR

GLY

ILE

11

HIS

TYR

ASN

GLU

ALA

ILE

GLU

PHE

CYS

12

GLN

ILE

LEU

ASP

LYS

LEU

ASN

ALA

VAL

13

LYS

Samples:

sample_1: rsa, [U-100% 13C; U-100% 15N], 1 mM; hit, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 150 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 950 MHz

PDB	2MJF 2MJF
DBJ	GAA26783 BAA01878 GAA24403
EMBL	CAA97906 CAY86767 CAA89583 CBK33766
GB	AHY77988 AJP41956 AJU23400 AJU24088 AJU24761 AAB39281 AAS56423 AHY79041 EDN63374 EDV12797
REF	NP_015131 NP_012589
SP	Q08932 P46973
TPG	DAA11241 DAA08842
AlphaFold	Q08932 P46973