BMRB Entry 19626

Title:
1H,13C and 15N assignment of Rsa1p(317-352)/Hit1p((70-164)   PubMed: 25170085
Deposition date:
2013-11-21
Original release date:
2014-09-08
Authors:
Quinternet, Marc; Roth, Benjamin; Back, Regis; Jacquemin, Clemence; Manival, Xavier
Citation:

Citation: Rothe, Benjamin; Saliou, Jean-Michel; Quinternet, Marc; Back, Regis; Tiotiu, Decebal; Jacquemin, Clemence; Loegler, Christine; Schlotter, Florence; Pena, Vlad; Eckert, Kelvin; Morera, Solange; Dorsselaer, Alain Van; Branlant, Christiane; Massenet, Severine; Sanglier-Cianferani, Sarah; Manival, Xavier; Charpentier, Bruno. "Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction."  Nucleic Acids Res. 42, 10731-10747 (2014).

Assembly members:

Assembly members:
rsa, polymer, 63 residues, Formula weight is not available
hit, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pnEA

Experimental source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pnEA

Data sets:
Data typeCount
13C chemical shifts596
15N chemical shifts149
1H chemical shifts970

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rsa1
2hit2

Entities:

Entity 1, rsa 63 residues - Formula weight is not available

1   GLYPROHISMETPHEALAASNGLUASNSER
2   GLNLEULEUASPPHEILEARGGLULEUGLY
3   ASPVALGLYLEULEUGLUTYRGLULEUSER
4   GLNGLNGLULYSASPVALLEUPHEGLYSER
5   SERGLUASPASNASNLYSASNHISTYRLYS
6   PROASNTYRLYSASNARGLYSPROASNLEU
7   SERARGALA

Entity 2, hit 122 residues - Formula weight is not available

1   METSERGLUGLNPROARGALAGLYTHRGLU
2   ALAASNVALGLUVALVALASNASNASPLYS
3   ILEILEASNSERSERLEUALAMETASNLYS
4   THRLEULYSTHRLYSALAPHEASPASPILE
5   TYRGLNASNSERALAGLULEUGLNGLULEU
6   LEULYSTYRASNTHRVALLYSPHEHISLEU
7   ALALYSVALTYRARGILELEUSERSERTHR
8   VALASNASPGLYSERSERGLYLYSMETASN
9   SERASPLEUGLNLYSGLULEUALAVALASN
10   TYRLEUASNTHRLEUARGTYRGLYGLYILE
11   HISTYRASNGLUALAILEGLUGLUPHECYS
12   GLNILELEULEUASPLYSLEUASNALAVAL
13   LYSLYS

Samples:

sample_1: rsa, [U-100% 13C; U-100% 15N], 1 mM; hit, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 150 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB
DBJ GAA26783 BAA01878 GAA24403
EMBL CAA97906 CAY86767 CAA89583 CBK33766
GB AHY77988 AJP41956 AJU23400 AJU24088 AJU24761 AAB39281 AAS56423 AHY79041 EDN63374 EDV12797
REF NP_015131 NP_012589
SP Q08932 P46973
TPG DAA11241 DAA08842
AlphaFold Q08932

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks