BMRB Entry 19617

Title:
Solution structure of the Big domain from Leptospira interrogans
Deposition date:
2013-11-17
Original release date:
2013-12-09
Authors:
Mei, Song
Citation:

Citation: Mei, Song. "Solution structure of the Big domain from Leptospira interrogans"  .

Assembly members:

Assembly members:
entity, polymer, 96 residues, 9481.341 Da.

Natural source:

Natural source:   Common Name: spirochetes   Taxonomy ID: 173   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Leptospira interrogans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Leptospira interrogans   Vector: pet22b

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts92
1H chemical shifts457

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Big domain from Leptospira interrogans1

Entities:

Entity 1, Big domain from Leptospira interrogans 96 residues - 9481.341 Da.

1   METPROALAALALEUVALSERILESERVAL
2   SERPROTHRASNSERTHRVALALALYSGLY
3   LEUGLNGLUASNPHELYSALATHRGLYILE
4   PHETHRASPASNSERASNSERASPILETHR
5   ASPGLNVALTHRTRPASPSERSERASNTHR
6   ASPILELEUSERILESERASNALASERASP
7   SERHISGLYLEUALASERTHRLEUASNGLN
8   GLYASNVALLYSVALTHRALASERILEGLY
9   GLYILEGLNGLYSERTHRASPPHELYSVAL
10   THRGLNALALEUGLUHIS

Samples:

sample_1: ammonium chloride, [U-99% 15N], 0.5 g/L; glucose, [U-95% 13C], 2.5 g/L; H2O 90%; D2O 10%; entity, [U-13C; U-15N], 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 25600 25819
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks