BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19601

Title: Solution structure of CXCL5   PubMed: 24695525

Deposition date: 2013-11-04 Original release date: 2014-03-31

Authors: Sepuru, Krishna Mohan; Rajarathnam, Krishna

Citation: Sepuru, Krishna Mohan; Poluri, Krishna Mohan; Rajarathnam, Krishna. "Solution Structure of CXCL5 - A Novel Chemokine and Adipokine Implicated in Inflammation and Obesity."  PLoS ONE 9, e93228-e93228 (2014).

Assembly members:
entity, polymer, 78 residues, 7830.323 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli' 'E. coli' . 562 Escherichia coli . . . . . . . . . . . . . . . . 'pET32 Xa/LIC

Entity Sequences (FASTA):
entity: AGPAAAVLRELRCVCLQTTQ GVHPKMISNLQVFAIGPQCS KVEVVASLKNGKEICLDPEA PFLKKVIQKILDGGNKEN

Data sets:
Data typeCount
13C chemical shifts138
15N chemical shifts70
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21

Entities:

Entity 1, entity_1 78 residues - 7830.323 Da.

1   ALAGLYPROALAALAALAVALLEUARGGLU
2   LEUARGCYSVALCYSLEUGLNTHRTHRGLN
3   GLYVALHISPROLYSMETILESERASNLEU
4   GLNVALPHEALAILEGLYPROGLNCYSSER
5   LYSVALGLUVALVALALASERLEULYSASN
6   GLYLYSGLUILECYSLEUASPPROGLUALA
7   PROPHELEULYSLYSVALILEGLNLYSILE
8   LEUASPGLYGLYASNLYSGLUASN

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate' 'natural abundance; .; .; .

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

SPARKY, Goddard - data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAG35064 BAJ20338
EMBL CAA55355 CAC42884 CAG33709
GB AAA62475 AAA86426 AAH08376 AAK29641 AAP35453
PRF 2104290A
REF NP_002985 XP_002814910 XP_003832377 XP_004038864
SP P42830

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts