BMRB Entry 19598

Name: 1H, 13C, and 15N Chemical Shift Assignments for human EPRS WHEP domains
Published: 2014-02-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19598

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for human EPRS WHEP domains

Deposition date:

2013-11-04

Original release date:

2014-02-11

Authors:

Shin, ChinHo

Citation:

Citation: Shin, Chinho; Hwang, Geum-Sook; Ahn, Hee-Chul; Kim, Sunghoon; Kim, Key-Sun. "(1)H, (13)C and (15)N resonance assignment of WHEP domains of human glutamyl-prolyl tRNA synthetase." Biomol. NMR Assignments 9, 25-30 (2015).
PubMed: 24378977

Assembly members:

Assembly members:
human_EPRS_WHEP_domains, polymer, 208 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_EPRS_WHEP_domains: DSLVLYNRVAVQGDVVRELK AKKAPKEDVDAAVKQLLSLK AEYKEKTGQEYKPGNPPAEI GQNISSNSSASILESKSLYD EVAAQGEVVRKLKAEKSPKA KINEAVECLLSLKAQYKEKT GKEYIPGQPPLSQSSDSSPT RNSEPAGLETPEAKVLFDKV ASQGEVVRKLKTEKAPKDQV DIAVQELLQLKAQYKSLIGV EYKPVSAT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	770
15N chemical shifts	211
1H chemical shifts	1299

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human EPRS WHEP domains	1

Entities:

Entity 1, human EPRS WHEP domains 208 residues - Formula weight is not available

1

ASP

SER

LEU

VAL

LEU

TYR

ASN

ARG

VAL

ALA

2

VAL

GLN

GLY

ASP

VAL

ARG

GLU

LEU

LYS

3

ALA

LYS

ALA

PRO

LYS

GLU

ASP

VAL

ASP

4

ALA

VAL

LYS

GLN

LEU

SER

LEU

LYS

5

ALA

GLU

TYR

LYS

GLU

LYS

THR

GLY

GLN

GLU

6

TYR

LYS

PRO

GLY

ASN

PRO

ALA

GLU

ILE

7

GLY

GLN

ASN

ILE

SER

ASN

SER

ALA

8

SER

ILE

LEU

GLU

SER

LYS

SER

LEU

TYR

ASP

9

GLU

VAL

ALA

GLN

GLY

GLU

VAL

ARG

10

LYS

LEU

LYS

ALA

GLU

LYS

SER

PRO

LYS

ALA

11

LYS

ILE

ASN

GLU

ALA

VAL

GLU

CYS

LEU

12

SER

LEU

LYS

ALA

GLN

TYR

LYS

GLU

LYS

THR

13

GLY

LYS

GLU

TYR

ILE

PRO

GLY

GLN

PRO

14

LEU

SER

GLN

SER

ASP

SER

PRO

THR

15

ARG

ASN

SER

GLU

PRO

ALA

GLY

LEU

GLU

THR

16

PRO

GLU

ALA

LYS

VAL

LEU

PHE

ASP

LYS

VAL

17

ALA

SER

GLN

GLY

GLU

VAL

ARG

LYS

LEU

18

LYS

THR

GLU

LYS

ALA

PRO

LYS

ASP

GLN

VAL

19

ASP

ILE

ALA

VAL

GLN

GLU

LEU

GLN

LEU

20

LYS

ALA

GLN

TYR

LYS

SER

LEU

ILE

GLY

VAL

21

GLU

TYR

LYS

PRO

VAL

SER

ALA

THR

Samples:

sample_1: human EPRS WHEP domains, [U-95% 13C; U-90% 15N], 2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

Varian Unity 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks