BMRB Entry 19589

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19589

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Title:
NMR assignment of the RhoGAP domain from the Rgd1 protein of Saccharomyces cerevisiae
Deposition date:
2013-10-29
Original release date:
2014-11-10
Authors:
Martinez, Denis; Prouzet-mauleon, Valerie; Hugues, Michel; Claveres, Annie; Doignon, Francois; Odaert, Benoit
Citation:

Citation: Martinez, Denis; Prouzet-Mauleon, Valerie; Hugues, Michel; Doignon, Francois; Odaert, Benoit. "Assignment of 1H, 13C and 15N resonances and secondary structure of the Rgd1-RhoGAP domain"  Biomol. NMR Assign. 12, 129-132 (2018).
PubMed: 29280056

Assembly members:

Assembly members:
Rgd1p-RhoGAP_domain, polymer, 226 residues, 25692 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rgd1p-RhoGAP_domain: MISHIQTNNNMPPGVQKNFK TFGVPLESLIEFEQDMVPAI VRQCIYVIDKFGLDQEGIYR KSANVLDVSKLKEEIDKDPA NISMILPSKPHSDSDIYLVG SLLKTFFASLPDSVLPKALS SEIKVCLQIEDPTTRKNFMH GLIYNLPDAQYWTLRALVFH LKRVLAHEAQNRMNLRALCI IWGPTIAPANPDDANDVNFQ IMAMEVLLEVSDQAFEPELE HHHHHH

Data sets:
Data typeCount
13C chemical shifts853
15N chemical shifts210
1H chemical shifts1431

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RhoGAP1

Entities:

Entity 1, RhoGAP 226 residues - 25692 Da.

The His-tag involve residues 219 to 226.

1   METILESERHISILEGLNTHRASNASNASN
2   METPROPROGLYVALGLNLYSASNPHELYS
3   THRPHEGLYVALPROLEUGLUSERLEUILE
4   GLUPHEGLUGLNASPMETVALPROALAILE
5   VALARGGLNCYSILETYRVALILEASPLYS
6   PHEGLYLEUASPGLNGLUGLYILETYRARG
7   LYSSERALAASNVALLEUASPVALSERLYS
8   LEULYSGLUGLUILEASPLYSASPPROALA
9   ASNILESERMETILELEUPROSERLYSPRO
10   HISSERASPSERASPILETYRLEUVALGLY
11   SERLEULEULYSTHRPHEPHEALASERLEU
12   PROASPSERVALLEUPROLYSALALEUSER
13   SERGLUILELYSVALCYSLEUGLNILEGLU
14   ASPPROTHRTHRARGLYSASNPHEMETHIS
15   GLYLEUILETYRASNLEUPROASPALAGLN
16   TYRTRPTHRLEUARGALALEUVALPHEHIS
17   LEULYSARGVALLEUALAHISGLUALAGLN
18   ASNARGMETASNLEUARGALALEUCYSILE
19   ILETRPGLYPROTHRILEALAPROALAASN
20   PROASPASPALAASNASPVALASNPHEGLN
21   ILEMETALAMETGLUVALLEULEUGLUVAL
22   SERASPGLNALAPHEGLUPROGLULEUGLU
23   HISHISHISHISHISHIS

Samples:

sample_1: Rgd1p-RhoGAP domain, [U-13C; U-15N], 400 uM; Tris 20 mM; DTT 2 mM; NaN3 0.1 mM

sample_2: Rgd1p-RhoGAP domain, [U-13C; U-15N], 400 uM; Rgd1p-RhoGAP domain, [U-13C; U-15N], 300 uM; d11-Tris 20 mM; DTT 5 mM; NaN3 0.1 mM

sample_3: Rgd1p-RhoGAP domain, [U-15N], 250 uM; Tris 20 mM; DTT 5 mM; NaN3 1 mM

sample_4: Rgd1p-RhoGAP domain, [U-13C; U-15N], 700 uM; Tris 20 mM; DTT 5 mM; NaN3 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.35; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_4isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

SP P38339
AlphaFold D6VQQ7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks