BMRB Entry 19579

Title:
NMR structure of C-terminal domain from A. ventricosus minor ampullate spidroin (MiSp)
Deposition date:
2013-10-24
Original release date:
2014-08-25
Authors:
Otikovs, Martins; Jaudzems, Kristaps; Andersson, Marlene; Chen, Gefei; Landreh, Michael; Nordling, Kerstin; Kronqvist, Nina; Westermark, Per; Jornvall, Hans; Knight, Stefan; Ridderstrale, Yvonne; Holm, Lena; Meng, Qing; Chesler, Mitchell; Johansson, Jan; Rising, Anna
Citation:

Citation: Otikovs, Martins; Jaudzems, Kristaps; Andersson, Marlene; Chen, Gefei; Landreh, Michael; Nordling, Kerstin; Kronqvist, Nina; Westermark, Per; Jornvall, Hans; Knight, Stefan; Ridderstrale, Yvonne; Holm, Lena; Meng, Qing; Chesler, Mitchell; Johansson, Jan; Rising, Anna. "Carbonic Anhydrase Generates CO2 and H+ That Drive Spider Silk Formation Via Opposite Effects on the Terminal Domains"  Plos Biol. 12, e1001921-e1001921 (2014).
PubMed: 25093327

Assembly members:

Assembly members:
MiSp-CT, polymer, 125 residues, 11766.900 Da.

Natural source:

Natural source:   Common Name: Orbweaver spider   Taxonomy ID: 182803   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Araneus ventricosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET vector

Data sets:
Data typeCount
13C chemical shifts463
15N chemical shifts130
1H chemical shifts749

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MiSp-CT, 11
2MiSp-CT, 21

Entities:

Entity 1, MiSp-CT, 1 125 residues - 11766.900 Da.

Residues -5 to -1 represent a non-native expression tag

1   GLYSERGLYASNSERVALTHRSERGLYGLY
2   TYRGLYTYRGLYTHRSERALAALAALAGLY
3   ALAGLYVALALAALAGLYSERTYRALAGLY
4   ALAVALASNARGLEUSERSERALAGLUALA
5   ALASERARGVALSERSERASNILEALAALA
6   ILEALASERGLYGLYALASERALALEUPRO
7   SERVALILESERASNILETYRSERGLYVAL
8   VALALASERGLYVALSERSERASNGLUALA
9   LEUILEGLNALALEULEUGLULEULEUSER
10   ALALEUVALHISVALLEUSERSERALASER
11   ILEGLYASNVALSERSERVALGLYVALASP
12   SERTHRLEUASNVALVALGLNASPSERVAL
13   GLYGLNTYRVALGLY

Samples:

sample_1: Minor ampullate spidroin CT, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 20 mM; sodium azide 0.03%; D2O, [U-100% 2H], 8%; H2O 92%

sample_2: Minor ampullate spidroin CT, [U-99% 13C; U-99% 15N], 0.25 mM; Minor ampullate spidroin CT 0.25 mM; sodium phosphate 20 mM; sodium chloride 20 mM; sodium azide 0.03%; D2O, [U-100% 2H], 8%; H2O 92%

sample_conditions_1: ionic strength: 0.0688 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 13C,15N filtered 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

UNIO, T. Herrmann, F. Fiorito, J. Volk - peak picking

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AFV31615

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks