BMRB Entry 19569

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19569

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Title:
NMR chemical shift assignments of mouse Vps28 C-terminal domain
Deposition date:
2013-10-21
Original release date:
2014-02-12
Authors:
Peterson, Tabitha; Fowler, Andrew; Piper, Robert; Yu, Liping
Citation:

Citation: Peterson, Tabitha; Yu, Liping; Piper, Robert. "Backbone and side-chain NMR assignments for the C-terminal domain of mammalian Vps28."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 24366722

Assembly members:

Assembly members:
Vps28C, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151D

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Vps28C: GIDPFTMNLNRCIADVVSLF ITVMDKLRLEIRAMDEIQPD LRELMETMHRMSHLPPDFEG RQTVSQWLQTLSGMSASDEL DDSQVRQMLFDLESAYNAFN RFLHA

Data sets:
Data typeCount
13C chemical shifts433
15N chemical shifts105
1H chemical shifts712

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vps28C1

Entities:

Entity 1, Vps28C 105 residues - Formula weight is not available

The N-terminal residues GIDPFTM are derived from affinity purification tag

1   GLYILEASPPROPHETHRMETASNLEUASN
2   ARGCYSILEALAASPVALVALSERLEUPHE
3   ILETHRVALMETASPLYSLEUARGLEUGLU
4   ILEARGALAMETASPGLUILEGLNPROASP
5   LEUARGGLULEUMETGLUTHRMETHISARG
6   METSERHISLEUPROPROASPPHEGLUGLY
7   ARGGLNTHRVALSERGLNTRPLEUGLNTHR
8   LEUSERGLYMETSERALASERASPGLULEU
9   ASPASPSERGLNVALARGGLNMETLEUPHE
10   ASPLEUGLUSERALATYRASNALAPHEASN
11   ARGPHELEUHISALA

Samples:

sample_1: Vps28C, [U-98% 13C; U-98% 15N], 600 uM; sodium phosphate 40 mM; sodium chloride 50 mM; sodium azide 3 mM; DTT 5 mM

sample_2: Vps28C, [U-98% 13C; U-98% 15N], 600 uM; sodium phosphate 40 mM; sodium chloride 50 mM; sodium azide 3 mM; deuterated DTT 5 mM

sample_conditions_1: pH: 6.95; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRView v08.01.00.00, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance II 500 MHz
  • Bruker Avance II 800 MHz

Related Database Links:

EMBL Q9D1C8
DBJ BAB22945 BAC25042
GB AAF00499 AAH06485 AAH13535 AAH19321 AAI02079
REF NP_001030581 NP_001123964 NP_001253325 NP_001292597 NP_057292
SP B5DEN9 Q3T178 Q9D1C8 Q9UK41
AlphaFold B5DEN9 Q3T178 Q9D1C8 Q9UK41

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks