BMRB Entry 19568

Name: assignment of the transmembrane domain of insulin receptor in detergent micelles
Published: 2014-02-12

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PDB ID: 2mfr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19568
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

assignment of the transmembrane domain of insulin receptor in detergent micelles

Deposition date:

2013-10-19

Original release date:

2014-02-12

Authors:

Li, Qingxin; Wong, Yinglei; Kang, Congbao

Citation:

Citation: Li, Qingxin; Wong, Ying Lei; Kang, CongBao. "Solution structure of the transmembrane domain of the insulin receptor in detergent micelles." Biochim. Biophys. Acta 1838, 1313-1321 (2014).
PubMed: 24440425

Assembly members:

Assembly members:
ISU, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ISU: MTYFYVTDYLDVPSNIAKII IGPLIFVFLFSVVIGSIYLF LRKRQPDGPLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	136
15N chemical shifts	46
1H chemical shifts	98

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ISUR	1

Entities:

Entity 1, ISUR 57 residues - Formula weight is not available

1

MET

THR

TYR

PHE

TYR

VAL

THR

ASP

TYR

LEU

2

ASP

VAL

PRO

SER

ASN

ILE

ALA

LYS

ILE

3

ILE

GLY

PRO

LEU

ILE

PHE

VAL

PHE

LEU

PHE

4

SER

VAL

ILE

GLY

SER

ILE

TYR

LEU

PHE

5

LEU

ARG

LYS

ARG

GLN

PRO

ASP

GLY

PRO

LEU

6

GLU

HIS

Samples:

sample_1: ISU, [U-100% 15N], 0.2 – 0.4 mM; ISU, [U-100% 13C; U-100% 15N], 0.2 – 0.4 mM; sodium phosphate 20 mM; DPC 2%; DTT 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v4.1, Johnson, One Moon Scientific - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz

PDB	2MFR
DBJ	BAD92098
EMBL	CAA26096
GB	AAA39318 AAA41441 AAA59174 AAA59176 AAA59177
PRF	1104300A
REF	NP_000199 NP_001073285 NP_034698 NP_058767 XP_001094337
SP	P06213 P15127 P15208
TPG	DAA27957
AlphaFold	P06213 P15127 P15208